Molecular cloning systems based on Gateway® DNA recombination, restriction enzymes or blunt-end ligation.
EZShuttle™ BP/LR Recombination Cloning System
- Gateway®-based recombinant cloning system
Fast-Fusion™ Cloning Kit
- For rapid and effective cloning of PCR products at restriction sites
NEW Smart-Join™ Blunt-end PCR Cloning Kit
- High cloning rate for blund-end PCR products
Competent cells
Suitable for many standard cloning applications, GeneCopoeia’s chemically competent cells provide high efficiency transformation. The cells enable fast screening with blue/white selection, ensure high quality plasmid preparations and contain recA1 endA1 mutations to increase insert stability. Available in single tube format, GeneCopoeia’s competent cells deliver a reliable platform for producing plasmid DNA for lentiviral and non-viral vector-based constructs.
UltraHiPF® DNA Polymerase Kit
UltraHiPF® DNA Polymerase is a heat-resistant ultra-fidelity DNA polymerase with 5′-3′ polymerase activity and 3′-5′ exonuclease activity. The polymerase can amplify uracil- and hypoxanthine- containing templates, GC- or AT- rich templates, genomes as low as one copy, and DNA fragments of more than 12 kb from different species. UltraHiPF® DNA Polymerase can specifically amplify 2 kb fragments at 1sec denaturation, annealing, and elongation, and can expand complex templates at 15-30 sec/kb. With Sanger sequencing, the mutation rate of this enzyme was 55 times lower than that of Taq DNA polymerase. The PCR product obtained by using the product has the Blunt-end.
2× UltraHiPF® PCR Mix
2× UltraHiPF® PCR Mix is a 2× premixed solution containing UltraHiPF® DNA Polymerase, dNTPs, and all other components required for PCR, excluding DNA templates and primers. The premixed solution saves time and reduces contamination by reducing the number of pipetting steps required to prepare the PCR reaction system.
NileHiFi® Long Amplicon PCR Kit
GeneCopoeia’s NileHiFi® Long Amplicon PCR Kit allows you to amplify templates up to 18kb of human genomic DNA (gDNA), 30kb of λ phage DNA, and other high molecular weight genomic DNA. This kit is recommended for amplifying GC rich or complex targets. The NileHiFi® Long Amplicon PCR Kit is the ideal choice for generating long PCR products with high sensitivity and processivity.