Smart-Join™ Blunt-end PCR Cloning Kit

Introduction

The Smart-Join™ Blunt-end PCR Cloning Kit offers a convenient method for high efficiency cloning of blunt-end PCR products (or any blunt-end DNA fragment). In the Smart-Join™ Blunt-end PCR Cloning Kit, successful ligation of a blunt-end PCR fragment to the linear vector disrupts the expression of Barnase, a small RNase toxic to cells, permitting growth of only positive recombinants upon transformation. Therefore, only the cells with target fragments ligated to the plasmids can grow. The kit provides high successful cloning rates and a more straightforward way to eliminate false positive transformant colonies that makes blue/white screening unnecessary.

Advantages

High successful cloning rate for blunt-end DNA fragments
Higher positive ligation rate and true positive colonies by the elimination of self-ligating products
Cloning of blund-end PCR products without additional sequences or restriction sites

Product Details

Smart-Join™ Blunt-end PCR Cloning Kit Work Flow

Figure 1. Smart-Join™ Blunt-end PCR Cloning Kit Performance Comparison. A variety of PCR products of different lengths were subjected to ligation reactions and transformed onto agar plates, the total number of colonies and the positive ligation rate were detected. It is shown that the ligation efficiency of Smart-Join™ Blunt-end PCR Cloning Kit (GeneCopoeia, Cat.No IC007) is comparable to that of Zero Blunt™ PCR Cloning Kit (Thermo Fisher, Cat.No K275020).