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Immortalized Cell Lines

Immortalized cell lines are derived from primary cells that bypass normal cellular senescence and have an extended life span. The primary cells can also be immortalized by over-expression of various viral genes like the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT). 

Immortalized cells are a highly useful tool for research in cell biology as they retain some characteristics of primary cells, thus providing an unlimited source of homogeneous cells for the research studies.

Advantages:

  • Easier to culture
  • Cost-effective
  • Better characterized, theoretically identical populations that bypass ethical concerns associated with the use of animal and human tissue
  • Grow quickly and can be used for the extraction of large amounts of proteins for biochemical assays

The immortalized cell lines are propagated for more than twenty passages to confirm immortalization and are also tested for sterility. Verification of transgene expression in immortalized cells is done by either immunostaining (Figure 1.) or qRT-PCR. 

   

Figure 1. Immunostaining of SV40-immortalized cells (middle, red) was done to confirm the cellular immortalization. The control, unstained cells were shown as a bright field image (left) and DAPI was used to stain nuclei (right, blue). All the images were taken at 40X magnification. The exposure time was 50 ms.

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SL421

Human gall bladder epithelial cell

SV40 large T antigen

$1395 $5495
SL422

Human skin fibroblasts

SV40 large T antigen

$1395 $5495
SL423

Human carotid artery-derived smooth muscle cell

SV40 large T antigen

$1395 $5495

SL427

Human bone marrow-derived mesenchymal stem cell

SV40 large T antigen

$1395 $5495
SL428

Human bone marrow-derived mesenchymal stem cell

SV40 large T antigen and hTERT gene

$1695 $5695
SL430
Human ectopic endometrium
epithelium cells
SV40 large T antigen $1395 $5495
SL431

Human adipose-derived mesenchymal stem cell

SV40 large T antigen

$1395 $5495
SL433

Human umbilical cord-derived mesenchymal stem cell

SV40 large T antigen

$1395 $5495
SL434

Human umbilical cord-derived mesenchymal stem cell

SV40 large T antigen and hTERT gene

$1695 $5695

 

Disclaimer: Pricing for premade cell lines are for customers in the US and Canada only. International customers, please contact your local distributor for pricing.

To obtain the datasheet for each cell line, please click on the designated catalog number.

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