Cell-Based Validation Services

– To validate ORF cDNA expression, shRNA knockdown, miRNA target or promoter activity

GeneCopoeia provides cell-based validation services to help researchers to validate the function and performance of expression clones using standard cell lines or customer’s own cell lines.

No matter you are studying transgene overexpression (ORF cDNA clones), gene knockdown (shRNA clones), transcription regulation in the promoter region (promoter reporter clones), or translation regulation by microRNA (microRNA target clones), GeneCopoeia’s expression clone collections and cell-based validation services are powerful tools to support your functional genomic research.

GeneCopoeia’s cell-based validation services cover ORF cDNA clone expression validation, shRNA knockdown validation, miRNA target validation and promoter activity validation.

Advantages

Largest clone collections to best suit your needs
Strict and rigorous experiment protocol to ensure quality results
Detailed reports and datasheets to assist decision making
Complete services to support your research needs

GeneCopoeia’s cell-based validation services include:

ORF cDNA clone expression validation	shRNA knockdown validation	miRNA target validation	Promoter activity validation
Detect recombinant protein expression in mammalian cells or bacteria using western blot.	Measure the efficiency of shRNA knockdown of GOI on the messenger-RNA level using qRT-PCR.	Validate the inhibitory effect of a particular miRNA on the 3’UTR region of GOI using luciferase reporter assays.	Detect exogenous promoter activation in mammalian cells using luciferase reporter assays.

Largest clone collections to best suit your needs
OmicsLink™ Expression-ready ORF cDNA Clones	OmicsLink™ shRNA clone collections	miTarget™ 3’UTR miRNA Target Clones miExpress™ Precursor miRNA Expression Clones miArrest™ miRNA Inhibitors	Gluc-ON™ Promoter Reporter Clones

Examples

ORF

Method: Western Blot

Figure 1. Secreted TPP1 recombinant protein expression in 293 cells.TPP1 protein was secreted into the culture medium 48hrs after transient transfection with TPP1 expression clone (Z5873). The culture media (15uL per lane) were collected, resolved using10% SDS-PAGE/Tris-Glycine, transferred to a NC membrane, and probed with the anti-Flag antibody (CGAB-DDK-0050). A) The NC membrane was stained with Ponceau S. B) The western blot results were visualized with a chromogenic substrate. The culture medium of HEK293T cells without transfection was used as the negative control (lane 1).