iPS cells are a type of pluripotent stem cell artificially derived, and often referred to as programmed, from adult somatic cells using the expression of certain genes in culture. Using adult somatic cells, they have been reprogrammed to an embryonic stem cell-like state by being forced to express genes and factors important for maintaining undifferentiated embryonic stem cells.
GeneCopoeia has several tools and related reagents that are useful to the stem cell scientist interested to perform reprogramming trials. More detailed information is found on the product-specific web page or submit your inquiry here.
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The OmicsLink™ expression-ready clone family is the most extensive and complete source of standard and specialized plasmid constructs anywhere. GeneCopoeia has several genes used in reprogramming available in many vectors with a variety of fusion tags. If your clone search doesn't find what you are looking for, send us an email at inquiry@genecopoeia.com.
| Click on product ID to view the vector options available for these genes |  |
| Accession | Symbol | Species | Description | Product ID |
|---|---|---|---|---|
| Z11898 | POU5F1 | Human | POU class 5 homeobox 1 | Z0092 |
| BC013923 | SOX2 | Human | SRY (sex determining region Y)-box 2 | T2547 |
| NM_002467 | MYC | Human | v-myc myelocytomatosis viral oncogene homolog (avian) | B0255 |
| NM_002467 | MYC | Human | v-myc myelocytomatosis viral oncogene homolog (avian) | Z2845 |
| NM_004235 | KLF4 | Human | Kruppel-like factor 4 (gut) | Z5703 |
| NM_024865 | NANOG | Human | Nanog homeobox | Z8289 |
| NM_024674 | LIN28 | Human | lin-28 homolog (C. elegans) | V1435 |
| NM_013633 | Pou5f1 | Mouse | Mus musculus POU domain, class 5, transcription factor 1 (Pou5f1), mRNA. | Mm24188 |
| NM_011443 | Sox2 | Mouse | Mus musculus SRY-box containing gene 2 (Sox2), mRNA. | Mm30354 |
| NM_010849 | Myc | Mouse | Mus musculus myelocytomatosis oncogene (Myc), mRNA. | Mm23755 |
| NM_010637 | Klf4 | Mouse | Mus musculus Kruppel-like factor 4 (gut) (Klf4), mRNA. | Mm19461 |
| NM_028016 | Nanog | Mouse | Mus musculus Nanog homeobox (Nanog), mRNA. | Mm23751 |
| NM_145833 | Lin28 | Mouse | Mus musculus lin-28 homolog (C. elegans) (Lin28), mRNA. | Mm12303 |
Learn more about expression-ready ORF cDNA clones.
For every gene you want to down regulate through RNA interference, GeneCopoeia uses a proprietary algorithm to carefully prepare four sequence-specific shRNA constructs. These constructs are available in lentiviral or non-viral vectors with a choice of eGFP or mCherry reporter gene. GeneCopoeia guarantee that at least one of the four constructs will give you 70% knockdown when tested with qRT-PCR or we will replace it with a new one.
| Click on product ID to view the vector options available for these genes | |
| Accession | Symbol | Species | Description | Product ID |
|---|---|---|---|---|
| NM_002701 | POU5F1 | Human | shRNA clone sets targeting NM_002701 | HSH105033 |
| NM_203289 | POU5F1 | Human | shRNA clone sets targeting NM_203289 | HSH105034 |
| NM_003106 | SOX2 | Human | shRNA clone sets targeting NM_003106 | HSH110118 |
| NM_002467 | MYC | Human | shRNA clone sets targeting NM_002467 | HSH117877 |
| NM_004235 | KLF4 | Human | shRNA clone sets targeting NM_004235 | HSH116341 |
| NM_024865 | NANOG | Human | shRNA clone sets targeting NM_024865 | HSH112480 |
| NM_024674 | LIN28 | Human | shRNA clone sets targeting NM_024674 | HSH112163 |
| NM_013633 | Pou5f1 | Mouse | shRNA clone sets targeting NM_013633 | MSH094318 |
| NM_011443 | Sox2 | Mouse | shRNA clone sets targeting NM_011443 | MSH095111 |
| NM_010849 | Myc | Mouse | shRNA clone sets targeting NM_010849 | MSH029723 |
| NM_010637 | Klf4 | Mouse | shRNA clone sets targeting NM_010637 | MSH054570 |
| NM_028016 | Nanog | Mouse | shRNA clone sets targeting NM_028016 | MSH104657 |
| NM_145833 | Lin28 | Mouse | shRNA clone sets targeting NM_145833 | MSH038608 |
Learn more about expression-ready shRNA clone collection.
GeneCopoeia also offers validated qRT-PCR primers for iPSC genes.