GeneCopoeia's Safe Harbor Knock-in ORF clones are designed for transferring the ORF of your gene of interest from a donor plasmid into the safe harbor sites in the human or mouse genomes via CRISPR or TALEN-mediated homologous recombination (HR; Figure 1). Over 20,000 sequence-verified human ORFs and over 15,000 mouse ORFs are available. Safe Harbor Knock-in ORF clones are designed for use with GeneCopoeia's Safe Harbor Gene Knock-in Kits
Human and Mouse Safe Harbor Knock-in Kits
Human AAVS1 and mouse ROSA26 safe harbor knock-in kits – CRISPR- or TALEN-mediated gene targeting kits designed to specifically transfer your gene of interest, selection marker or other genetic element into the human AAVS1 or mouse safe harbor sites. More than 35,000 AAVS1- and ROSA26-compatible ORF donor clones are also available. The safe integration ensures transcription-competency of the transgenes and presents no known adverse effects on fitness.
To display your kits of interest, select species, genome editing technology, and donor options from the table below.
Technology Overview
Safe Harbor Knock-in ORF Clones
Figure 1. Genome-editing-tool-mediated transgene integration at the safe harbor sites
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Figure 2. Maps of (A) Human safe harbor AAVS1 knock-in ORF clone and (B) Mouse safe harbor ROSA26 knock-in ORF clone
Visit the Safe Harbor knock-in kits page for more information.
Safe Harbor Knock-in ORF Clones
Product Details
Figure 3: Human genome safe harbor AAVS1 gene targeting of OCT4 and SOX2.
(A) OCT4 (Cat#: DC-Z0092-SH01) or SOX2 (Cat#: DC-T2547-SH01) donor plasmids were co-transfected with an AAVS1 TALEN pair into HEK293T cells in a 6-well pate. Cells were sub-cultured 48 hr post-transefection and selected against puromycin (1 µg/ml) for 2 weeks. The expression of copGFP was checked under a microscope (Nikon Eclipse Ti ) 48h post-transfection or after 2 weeks of drug selection. Few colonies remain from cells transfected with donor plasmid alone after puromycin selection (data not shown).
(B) Western blot analysis of proteins from HEK293T cells stably integrated with SOX2 (Cat#: DC-T2547-SH01) or OCT4 (Cat#: DC-Z0092-SH01) at the AAVS1 site, with cells alone as negative control. Endogenous Sox2 and OCT4 protein levels were too low to be detected in the same blot.
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