Human and Mouse Safe Harbor Knock-in ORF cDNA Clones and Kits

GeneCopoeia’s Safe Harbor Knock-in ORF cDNA clones are designed for transferring the ORF cDNA of your gene of interest from a donor plasmid into the safe harbor sites in the human or mouse genomes via CRISPR or TALEN-mediated homologous recombination (HR; Figure 1). Over 20,000 sequence-verified human ORF cDNAs and over 15,000 mouse ORF cDNAs are available. Safe Harbor Knock-in ORF cDNA clones are designed for use with GeneCopoeia’s Safe Harbor Gene Knock-in Kits
Knock-In kits

Human and Mouse Safe Harbor Knock-in Kits

Human AAVS1 and mouse ROSA26 safe harbor knock-in kits – CRISPR- or TALEN-mediated gene targeting kits designed to specifically transfer your gene of interest, selection marker or other genetic element into the human AAVS1 or mouse ROSA26 safe harbor sites. More than 35,000 AAVS1- and ROSA26-compatible ORF cDNA donor clones are also available. The safe integration ensures transcription-competency of the transgenes and presents no known adverse effects on fitness.

 

To display your kits of interest, select species, genome editing technology, and donor options from the table below. 

Species

CRISPR or TALEN

Donor

Buy Catalog # Product Description Price (US$) Cas9 clone User Manual
SH004 GeneHero™ human AAVS1 safe harbor gene knock-in kit CRISPR-based cloning kit for human AAVS1 Safe Harbor knockin. Includes CRISPR-Cas9 clone targeting AAVS1,donor cloning vector,control RFP knockin clone, and verification PCR primer pairs $1395
SH000 GeneHero™ human AAVS1 safe harbor gene knock-in kit (without donor) CRISPR-based kit for human AAVS1 Safe Harbor knockin. Includes CRISPR-Cas9 clone targeting AAVS1, control RFP knockin clone, and verification PCR primer pairs $1195
SH029 Genome-TALER™ human AAVS1 safe harbor gene knock-in kit TALEN-based cloning kit for human AAVS1 Safe Harbor knockin. Includes TALEN pair targeting AAVS1,donor cloning vector,control RFP knockin clone, and verification PCR primer pairs $1395
SH054 GeneHero™
mouse ROSA26 safe harbor gene knock-in kit
CRISPR-based cloning kit for mouse ROSA26 Safe Harbor knockin. Includes CRISPR-Cas9 clone targeting ROSA26, donor cloning vector, control RFP knockin clone, and verification PCR primer pairs $1395
SH079 Genome-TALER™
mouse ROSA26 safe harbor gene knock-in kit
TALEN-based cloning kit for mouse ROSA26 Safe Harbor knockin. Includes TALEN pair targeting ROSA26,donor cloning vector,control RFP knockin clone, and verification PCR primer pairs $1395
SH012 GeneHero™ Cas9 human AAVS1 Safe Harbor knockin kit-Puro CRISPR-based kit for knockin of Cas9 nuclease to human AAVS1 Safe Harbor site-puromycin selection. Includes CRISPR-Cas9 clone targeting AAVS1, Cas9-AAVS1 donor clone with puromycin selection, and verification PCR primer pairs. $1595 DC-C9NU-03
SH013 GeneHero™ Cas9 human AAVS1 Safe Harbor knockin kit-Hygro CRISPR-based kit for knockin of Cas9 nuclease to human AAVS1 Safe Harbor site-hygromycin selection. Includes CRISPR-Cas9 clone targeting AAVS1, Cas9-AAVS1 donor clone with hygromycin selection, and verification PCR primer pairs $1595 DC-C9NU-04
SH014 GeneHero™ Cas9 human AAVS1 Safe Harbor knockin kit-Neo CRISPR-based kit for knockin of Cas9 nuclease to human AAVS1 Safe Harbor site-neomycin selection. Includes CRISPR-Cas9 clone targeting AAVS1, Cas9-AAVS1 donor clone with neomycin selection, and verification PCR primer pairs $1595 DC-C9NU-05
SH037 Genome-TALER™ Cas9 human AAVS1 Safe Harbor knockin kit-Puro TALEN-based kit for knockin of Cas9 nuclease to human AAVS1 Safe Harbor site-puromycin selection. Includes TALEN clones targeting AAVS1, Cas9-AAVS1 donor clone with puromycin selection, and verification primer pairs $1595 DC-C9NU-03
SH038 Genome-TALER™ Cas9 human AAVS1 Safe Harbor knockin kit-Hygro TALEN-based kit for knockin of Cas9 nuclease to human AAVS1 Safe Harbor site-hygromycin selection. Includes TALEN clones targeting AAVS1, Cas9-AAVS1 donor clone with hygromycin selection, and verification primer pairs $1595 DC-C9NU-04
SH039 Genome-TALER™ Cas9 human AAVS1 Safe Harbor knockin kit-Neo TALEN-based kit for knockin of Cas9 nuclease to human AAVS1 Safe Harbor site-neomycin selection. Includes TALEN clones targeting AAVS1, Cas9-AAVS1 donor clone with neomycin selection, and verification primer pairs $1595 DC-C9NU-05
SH062 GeneHero™ Cas9 mouse ROSA26 Safe Harbor knockin kit-Puro CRISPR-based kit for knockin of Cas9 nuclease to Mouse ROSA26 Safe Harbor site-puromycin selection. Includes CRISPR-Cas9 clone targeting ROSA26, Cas9-ROSA26 donor clone with puromycin selection, and verification PCR primer pairs $1595 DC-C9NU-06
SH063 GeneHero™ Cas9 mouse ROSA26 Safe Harbor knockin kit-Hygro CRISPR-based kit for knockin of Cas9 nuclease to mouse ROSA26 Safe Harbor site-hygromycin selection. Includes CRISPR-Cas9 clone targeting ROSA26, Cas9-ROSA26 donor clone with hygromycin selection, and verification PCR primer pairs $1595 DC-C9NU-07
SH064 GeneHero™ Cas9 mouse ROSA26 Safe Harbor knockin kit-Neo CRISPR-based kit for knockin of Cas9 nuclease to mouse ROSA26 Safe Harbor site-neomycin selection. Includes CRISPR-Cas9 clone targeting ROSA26, Cas9-ROSA26 donor clone with neomycin selection, and verification PCR primer pairs $1595 DC-C9NU-08
SH087 Genome-TALER™ Cas9 mouse ROSA26 Safe Harbor knockin kit-Puro TALEN-based kit for knockin of Cas9 nuclease to mouse ROSA26 Safe Harbor site-puromycin selection. Includes TALEN clones targeting ROSA26, Cas9-ROSA26 donor clone with puromycin selection, and verification PCR primer pairs $1595 DC-C9NU-06
SH088 Genome-TALER™ Cas9 mouse ROSA26 Safe Harbor knockin kit-Hygro TALEN-based kit for knockin of Cas9 nuclease to mouse ROSA26 Safe Harbor site-hygromycin selection Includes TALEN clones targeting ROSA26, Cas9-ROSA26 donor clone with hygromycin selection, and verification PCR primer pairs $1595 DC-C9NU-07
SH089 Genome-TALER™ Cas9 mouse ROSA26 Safe Harbor knockin kit-Neo TALEN-based kit for knockin of Cas9 nuclease to mouse ROSA26 Safe Harbor site-neomycin selection. Includes TALEN clones targeting ROSA26, Cas9-ROSA26 donor clone with neomycin selection, and verification PCR primer pairs $1595 DC-C9NU-08
Varies AAVS1 knockin ORF donor clone AAVS1 knockin ORF donor clone containing CMV-driven ORF of customer's gene of interest
Variable Mouse ROSA26 knockin ORF donor clones Mouse ROSA26 knock-in ORF donor clone containing CMV-driven ORF of customer's gene of interest
SH000 GeneHero™ human AAVS1 safe harbor gene knock-in kit
(without donor)
CRISPR-based kit for human AAVS1 Safe Harbor knockin. Includes CRISPR-Cas9 clone targeting AAVS1, control RFP knockin clone, and verification PCR primer pairs $1195
SH050 GeneHero™
mouse ROSA26 safe harbor gene knock-in kit
(without donor)
CRISPR-based kit for mouse ROSA26 Safe Harbor knockin. Includes CRISPR-Cas9 clone targeting ROSA26 and verification PCR primer pairs $1195
SH025 Genome-TALER™ human AAVS1 safe harbor gene knock-in kit(without donor) TALEN-based kit for human AAVS1 Safe Harbor knockin. Includes TALEN clone targeting AAVS1 and verification PCR primer pairs $1195
SH075 Genome-TALER™ mouse ROSA26 safe harbor gene knock-in kit (without donor) TALEN-based kit for mouse ROSA26 Safe Harbor knockin. Includes TALEN clone targeting ROSA26 and verification PCR primer pairs $1195
  varies AAVS1/ROSA26 knockin ORF donoe clone AAVS1/ROSA26 knockin ORF donoe clone containing CMV-driven ORf of customer's gene of interest
  varies AAVS1/ROSA26 knockin ORF donoe clone AAVS1/ROSA26 knockin ORF donoe clone containing CMV-driven ORf of customer's gene of interest
  varies AAVS1/ROSA26 knockin ORF donoe clone AAVS1/ROSA26 knockin ORF donoe clone containing CMV-driven ORf of customer's gene of interest
  varies AAVS1/ROSA26 knockin ORF donoe clone AAVS1/ROSA26 knockin ORF donoe clone containing CMV-driven ORf of customer's gene of interest