Human AAVS1 safe harbor gene knock-in kits and clones

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Introduction

AAVS1 (also known as the PPP1R12C locus) on human chromosome 19 is a well-validated “safe harbor” for hosting DNA transgenes with expected function. It has an open chromatin structure and is transcription-competent. Most importantly, there are no known adverse effects on cells resulting from the inserted DNA fragment of interest.

AAVS1-specific TALENs or CRISPR-Cas9 sgRNAs generate DNA double-strand breaks (DSBa) at the AAVS1 locus, stimulating DNA repair. In the presence of AAVS1 ORF knock-in clones, homologous recombination (HR) occurs and integrates the ORF knock-in into the safe harbor locus.

Human AAVS1 Safe Harbor knock-in kits

Buy Catalog # Product Description Price
SH029 Genome-TALER™ human AAVS1 safe harbor gene knock-in kit Includes: AAVS1 TALEN pair (TN-AAVS1) AAVS1 donor cloning vector (DC-DON-SH01) AAVS1 positive control donor (DC-RFP-SH01) knock-in verification primer pairs (HQPAVSHR) $1395
SH025 Genome-TALER™ human AAVS1 safe harbor gene knock-in kit (without donor) Includes: AAVS1 TALEN pair (TN-AAVS1) AAVS1 positive control (DC-RFP-SH01) knock-in verification primer pairs (HQPAVSHR) $1195
SH004 GeneHero™ human AAVS1 safe harbor gene knock-in kit Includes: AAVS1 All-in-one sgRNA/Cas9 expression clone (HCP-AAVS1-CG02) AAVS1 donor cloning vector (DC-DON-SH01) AAVS1 positive control donor (DC-RFP-SH01) knock-in verification primer pairs (HQPAVSHR) $1395
SH000 GeneHero™ human AAVS1 safe harbor gene knock-in kit (without donor) Includes: AAVS1 All-in-one sgRNA/Cas9 expression clone (HCP-AAVS1-CG02) AAVS1 positive control donor (DC-RFP-SH01) knock-in verification primer pairs (HQPAVSHR) $1195

Human AAVS1 Safe Harbor knock-in ORF clones

Catalog # Product Description Price
Varies AAVS1 knock-in ORF donor clone AAVS1 knock-in ORF donor clone containing CMV-driven ORF of customer’s gene of interest

Human AAVS1 Safe Harbor knock-in CRISPR-Cas9 clones

Important Note: The vector-specific knock-in verification primers provided with the Human AAVS1 Safe Harbor knockin kits (catalog numbers: SH029, SH025, SH004, and SH000) are not compatible with the Human AAVS1 Safe Harbor knock-in CRISPR-Cas9 clones in the table below. If you purchase these clones with our Human Safe Harbor knock-in kits, you will be provided with clone-specific knock-in verification primers.
 
Buy Catalog # Product Application Selection marker Promoter Cas9 integration site Delivery format Price
SH300 Cas9 AAVS1 knock-in donor clone To knock-in Cas9 nuclease to human AAVS1 Safe Harbor site using CRISPR or TALEN Puro CBh AAVS1 Bacterial stock or transfection-ready DNA $495
SH301 Cas9 AAVS1 knock-in donor clone Hygro CBh AAVS1 $495
SH302 Cas9 AAVS1 knock-in donor clone Neo CBh AAVS1 $495
 
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