2× UltraHiPF® PCR Mix is a 2× premixed solution containing UltraHiPF® DNA Polymerase, dNTPs, and all other components required for PCR, excluding DNA templates and primers. The premixed solution saves time and reduces contamination by reducing the number of pipetting steps required to prepare the PCR reaction system.
UltraHiPF® DNA Polymerase is a heat-stable, ultra-fidelity DNA polymerase with 5′-3′ polymerase activity and 3′-5′ exonuclease activity. The polymerase can amplify uracil- and hypoxanthine- containing templates, GC- or AT- rich templates, low copy genomes, and DNA fragments greater than 12 kb in length from various species. The enzyme has a 55-fold lower mutation rate than Taq DNA polymerase by Sanger sequencing. The PCR products obtained with the product have blunt ends.
Advantages- 55X higher fidelity than Taq
- Robust reactions – maximal success with minimal optimization
- Superior performance for a broad range of amplicons
