EZShuttle™ Recombination Cloning System

EZRecombinase™ LR Enzyme Mix
EZRecombinase™ LR Enzyme Mix
EZRecombinase™ LR Enzyme Mix
EZRecombinase™ LR Enzyme Mix
EZRecombinase™ BP Enzyme Mix
EZRecombinase™ BP Enzyme Mix
EZRecombinase™ BP Enzyme Mix
EZRecombinase™ BP Enzyme Mix
EZRecombinase™ BP II Enzyme Mix (-20&#8451)
EZRecombinase™ BP II Enzyme Mix (-20℃)
EZRecombinase™ BP II Enzyme Mix (-20&#8451)
EZRecombinase™ BP II Enzyme Mix (-20℃)

EZRecombinase™ LR Enzyme Mix EZRecombinase™ LR Enzyme Mix EZRecombinase™ BP Enzyme Mix EZRecombinase™ BP Enzyme Mix EZRecombinase™ BP II Enzyme Mix (-20℃) EZRecombinase™ BP II Enzyme Mix (-20℃)

Price: $203.00 $910.00 $203.00 $910.00 $203.00 $910.00
Catalog#:
  • ER001
  • ER002
  • ER003
  • ER004
  • ER013
  • ER014
Size: 20 rxn 100 rxn 20 rxn 100 rxn 20 rxn 100 rxn
EZRecombinase™ LR/BP Enzyme Mix – 30% Off*
Ends February 28, 2025
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-Efficient cloning system based on phage lambda recombination, the same principle as Gateway® technology.

*Promotions are valid in the US & Canada only. For international customers, please contact your local distributors. Promotion ends February 28, 2025. Discounts are not valid on previous purchases and cannot be combined with additional discounts.

Introduction
The GeneCopoeia EZShuttle™ cloning system uses the site-specific recombination machinery between the E. coli and phage lambda genomes for efficient DNA fragment transfer between plasmids, the same principle as Gateway® technology. The EZShuttle™ system includes the following major components. Click on each tab above for more details:

1. EZRecombinase™ Mixes for catalyzing attL x attR and attB x attP cloning reactions.

2. pEZ™ expression cloning vectors for creating ready-to-use expression clones.

3. EZShuttle™ Gateway® PLUS ORF clones. More than 35,000 human and mouse ORFs in pShuttle™ plasmid backbones.

The EZShuttle™ cloning system is fully compatible with Gateway® cloning, as shown in Table 1.

Table 1. Compatiblilty between components of GeneCopoeia’s EZShuttle™ recombination cloning system and Gateway® cloning technology.

Advantages

  • Complete system of recombination cloning enzymes, expression cloning vectors, and sequence-verified ORF  clones.
  • Highly active for EZRecombinase™ LR Mix and EZRecombinase™ BP Mix.
  • Choice of pEZ™ expression cloning vectors with a wide variety of selection markers and tags for cloning, processing, protein purification and gene function studies.
  • Compatible with existing Gateway® cloning systems.

Applications

EZShuttle™ recombination cloning provides a highly fascile system for many powerful applications, including:

  • Create ready-to-express expression clones. Readily shuttle ORFs or other DNA fragments of interest into an expression vector.
  • Protein purification. Express your gene of interest in one or more systems for protein purification, leading to crystallization, antibody generation, DNA binding assays, and more.
  • Promoter swapping. Transfer your gene of interest into vectors carrying different promoters.
  • Promoter analysis. Test the function of multiple promoter candidates with a reporter such as luciferase.
  • Gene tagging. Express your gene either untagged or with a multitude of in-frame fusion tags, such as 6XHis, Flag®, GFP, etc. Useful for co-immunoprecipitation, subcellular localization, etc.

Mechanism of EZShuttle™ cloning

GeneCopoeia’s EZShuttle™ recombination-based cloning system for DNA fragment transfer between plasmid vectors is based on the site-specific recombination machinery between the E. coli and phage lambda genomes, the same principle as Gateway® technology (Figure 1).

Figure 1. The attachment sites and proteins used for integration and excision reactions between E.coli and phage lambda genomes

Note: Gateway® is a registered trademark of Life Technologies.