EZShuttle™ Gateway® PLUS ORF Clones

A high value choice for both recombination and traditional cloning


EZShuttle™ Gateway® PLUS ORF clones offer both recombination and multiple cloning sites making them highly multifunctional and cost-effective. The collection consists of 20,000 human and 15,000 mouse EZShuttle™ Gateway® PLUS ORF clones.

EZShuttle™ Gateway® PLUS ORF clones are an integral part of GeneCopoeia’s EZShuttle™ recombination cloning system, and are also compatible with existing Gateway® systems, permitting easy and rapid transfer of protein-coding ORFs into compatible destination expression vectors.. These clones can then be used for expression and functional analysis of target proteins in E.coli, yeast, baculovirus, CHO and mammalian cell lines as well as cell-free transcription and translation coupling systems.



Features of EZShuttle™ Gateway® PLUS ORF Clones

  • Available with and without stop codons
  • Ribosomal binding sites (Shine Dalgarno and Kozak
    Sequence) for optimal translational context
  • Multiple cloning sites included


Figure 1: EZShuttle Gateway® PLUS ORF clones can be used for both recombination as well as traditional restriction digestion ligation cloning.

EZShuttle™ Gateway® PLUS provides more options    

  • Recombination cloning – The presence of attL1 and attL2 sites enables transfer via recombination of ORFs into any destination vector using recombination cloning technology.
  • More compatibility –  Unlike other recmbination cloning systems, EZShuttle™ Gateway® PLUS clones enable traditional restriction digestion ligation cloning. The full ORF of EZShuttle™ Gateway® PLUS clones have multiple cloning sites in the linker between attachment site (attL1 site) and start codon (ATG). This makes them compatible with many other cloning systems using the classical restriction-ligation cloning method.

Rigorous quality control and assurance process 

Human and mouse EZShuttle™ Gateway® PLUS ORF clones are derived from full-length human and mouse genes that were chosen through a strict selection process. This process includes the extraction, comparison as well as validation of gene sequences and annotation information from multiple public and private sources; clustering and reduction of redundant gene sequences, filtering out sequencing errors, plus several other manual curation steps.

Following this stringent selection process, the entire coding open reading frames (ORFs) were generated from sequence validated full-length cDNA clones or high-quality human tissue cDNA libraries and then inserted into a modified Gateway® entry vector using GeneCopoeia’s proprietary high – fidelity cloning technologies.

Once the clone is constructed, GeneCopoeia follows additional quality control steps to ensure the right clone is delivered to you.

  • All ORFs are fully sequenced.
  • PCR amplification and size validation
  • Enzyme digestion check of the integrity of whole plasmid.


Note: Gateway® is a registered trademark of ThermoFisher Scientific.