Resources

CRISPR-Cas9 Protein for Highly Efficient Genome Editing With Minimal Off-Target Activity

  Ed Davis, Ph.D. Introduction The Clustered, Regularly Interspaced, Short Palindromic Repeats (CRISPR) system is widely used for editing genes and modulating gene expression, thanks to its ease-of-design, efficiency, specificity, and relatively low cost (Wang,...

How To Choose a GeneCopoeia Luciferase System

  Ed Davis, Ph.D. Introduction Luciferase reporter systems are invaluable tools for several applications, including regulation of gene expression and high-throughput compound screening (reviewed in Thorne, et al., 2010). GeneCopoeia has developed several...

Choosing Between Lentivirus and Adeno-associated Virus For DNA Delivery

  Ed Davis, Ph.D. Introduction Lentivirus and Adeno-associated virus (AAV) have proven invaluable for introducing genetic material into mammalian cells, either in culture or whole animals. Both systems are highly amenable for many basic research applications,...

Cas9 Stable Cell Lines: Powerful Tools for CRISPR sgRNA Library Screening and More

  Ed Davis, Ph.D. Introduction The CRISPR-Cas9 system for genome editing is rapidly transforming research in biology and medicine, due to its ease-of-design, efficiency, specificity, and relatively low cost (Wang, et al., 2016). In mammalian cell culture systems,...