Custom CRISPR sgRNA Design and Cloning Services Inquiry Form

1.Contact information

Name :	*
Institution :	*
Institution e-mail :	*
	Please check if you Do Not wish to receive promotion and new product information
Telephone :	*

Species of interest *

	Human
	Mouse
	Rat
	Other species ( Please specify )

What’s your application?*

	Knockout
	Mutagenesis
	Gene tagging
	Insert a fragment
	Delete a fragment
	Swap a fragment
	Other. ( Please specify )

Enter the accession number and gene symbol of your target gene *

Enter the target region information (sequence or exon number) *

How many sites do you want to target in this genome *

	One
	Multiple

For multiple target sites, select the sgRNA cloning option:

	Each sgRNA is cloned into a single vector (more flexibility, but needs co-transfection)
	Multiple sgRNAs are cloned into one vector (less flexibility, but no need co-transfection)

Choose a vector backbone you prefer:

	Vector pCRISPR-SG01 (express sgRNA only)
	Vector pCRISPR-CG12 (express sgRNA and CMV-Cas9)
	Vector pCRISPR-CG02 (express sgRNA and CBh-Cas9)
	Lentiviral vector pCRISPR-LvSG03 (express sgRNA only)
	sgRNA pair design for Cas9 nickase using vector pCRISPR-SG01 (express sgRNA only)

Choose a vector backbone you prefer:

	Vector pCRISPR-SG01 (express sgRNA only)
	Vector pCRISPR-CG01 (express sgRNAs and Cas9)

7.Do you need a (donor clone

Donor clones are custom-designed plasmids used to introduce insertion, deletion, mutation, replacement, fusion tag, selection marker or other modifications into a specific chromosomal target site via homologous recombination (HR).

)?If yes, please choose the donor backbone you are interested in.*

	pDonor-D01(EFa1/copGFP/Puro)
	pDonor-D02(CMV/copGFP/Neo)
	pDonor-D03(CMV/Neo)
	pDonor-D04(CMV/Puro)
	pDonor-D05(EFa1/Neo)
	pDonor-D07(EFa1/copGFP/Puro/TK/LoxP)
	pDonor-D08(CMV/copGFP/Neo/TK/LoxP)
	pDonor-D09(EFa1/Puro/TK/LoxP)
	pDonor-D10(CMV/Neo/TK/LoxP)
	pDonor-D11(PGK/copGFP/Puro/TK/LoxP)
	pDonor-D12(EF1a/copGFP/Hygro/TK/LoxP)
	pDonor-D13(PGK/copGFP/Neo/TK/LoxP)
	pDonor-D14(PGK/Puro/TK/LoxP)
	Other. ( Please specify )

8.Do you need functional validations? *

	Chromosomal level functional validation service(? Detects the cleavage activity of CRISPR on a chromosomal target in mammalian cells. In certain circumstances, the naturally-occurred chromatin structure may interfere the CRISPR activity. )
	None

9. What would you like to do next after the construct is validated?

	I would like to have monoclonal cells containing single-allele modification
	I would like to have monoclonal cells containing double-allele modification
	Other.( Please specify )
	My cell type is

Other requirements and comment

Need more help? Please contact us at

Email:	inquiry@genecopoeia.com
Call:	866-360-9531 or 301-762-0888

Otherwise, please click Next to view the summary of your selections