Introduction
GeneCopoeia’s GLuc-ON™ PKC transcriptional response element (TRE) clone enables analysis of the effects on genes regulated by activator protein 1 (AP1). AP1 is a transcription factor complex comprised of the c-Fos and c-Jun family proteins, and is necessary for signaling through the Protein kinase C (PKC) pathway.
The GLuc-ON™ PKC TRE clone contains tandem repeats of an AP1 binding site upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The AP1 response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ PKC TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.
Figure 1. Activation of the GLuc-ON™ PKC TRE by treatment with phorbol 12-myristate 13-acetate (PMA). HEK293a cells were transfected with a negative control clone or the GLuc-ON™ PKC TRE clone. Transfected cells were incubated for 24 hours, then treated with different amounts of PKC for 24 hours. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as luminance fold activation.