PKC transcriptional response element (TRE) clone

GeneCopoeia's GLuc-ON™ PKC transcriptional response element (TRE) clone enables analysis of the effects on genes regulated by activator protein 1 (AP1). AP1 is a transcription factor complex comprised of the c-Fos and c-Jun family proteins,  and is necessary for signaling through the Protein kinase C (PKC) pathway.

The GLuc-ON™ PKC TRE clone contains tandem repeats of an AP1 binding site upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The AP1 response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ PKC TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.

 
Buy Cat.No. Product name Price
TR106 PKC TRE clone for PKC/MAPK signaling pathway $350
SEAP-PA01 Secreted alkaline phosphatase (SEAP) clone for normalization $395
TR001 TRE negative control clone $300
GAPDH-PG02 GAPDH positive control clone $375

 

 

Secrete-Pair Luminescence Assay Kits

Buy Catalog# Product Description Price
LF031 Secrete-Pair Dual Luminescence Assay Kit (100 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF032 Secrete-Pair Dual Luminescence Assay Kit (300 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF033 Secrete-Pair Dual Luminescence Assay Kit (1000 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF061 Secrete-Pair Gaussia Luciferase Assay Kit (100 rxns) Detects Gaussia luciferase (GLuc) inquire
LF062 Secrete-Pair Gaussia Luciferase Assay Kit (1000 rxns) Detects Gaussia luciferase (GLuc) inquire

 

EndoFectin transfection reagents

Buy Cat.No. Product name Price Data sheets
EFM1004-01 EndoFectin™ Max transfection reagent (1 mL) $295 Download
EFM1004-02 EndoFectin™ Max transfection reagent (3 mL) $589
EFL1001-01 EndoFectin™ Lenti transfection reagent (1 mL) $245 Download
EFL1001-02 EndoFectin™ Lenti transfection reagent (3 mL) $489
EF005 EndoFectin™ HepG2 transfection reagent (1 mL) $355 Download
EF006 EndoFectin™ HepG2 transfection reagent (3 mL) $705
EF007 EndoFectin™ Expi293 transfection reagent (1 mL) $320  
EF008 EndoFectin™ Expi293 transfection reagent (3 mL) $640  

 

Validation Data

Figure 1. Activation of the GLuc-ON™ PKC TRE by treatment with phorbol 12-myristate 13-acetate (PMA). HEK293a cells were transfected with a negative control clone or the GLuc-ON™ PKC TRE clone. Transfected cells were incubated for 24 hours, then treated with different amounts of PKC for 24 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminance fold activation.