PR transcriptional response element (TRE) Clone

GeneCopoeia's GLuc-ON™ PR transcriptional response element (TRE) clone enables analysis of the activity of signal transduction mediated by progesterone receptors (PR). Progesterone receptors are transcription factors that, in response to stimulation by progesterone, lead to proliferation of breast tissue. Aberrant function and/or expression of PRs is an important factor in development of breast cancer

The GLuc-ON™ PR TRE clone contains tandem repeats of PR upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The PR response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ PR TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.

 
Buy Cat.No. Product name Price
TR113 PR TRE clone for progesterone receptor signaling pathway $350
SEAP-PA01 Secreted alkaline phosphatase (SEAP) clone for normalization $395
TR001 TRE negative control clone $300
GAPDH-PG02 GAPDH positive control clone $375

 

 

Secrete-Pair Luminescence Assay Kits

Buy Catalog# Product Description Price
LF031 Secrete-Pair Dual Luminescence Assay Kit (100 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF032 Secrete-Pair Dual Luminescence Assay Kit (300 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF033 Secrete-Pair Dual Luminescence Assay Kit (1000 rxns) Detects Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP) inquire
LF061 Secrete-Pair Gaussia Luciferase Assay Kit (100 rxns) Detects Gaussia luciferase (GLuc) inquire
LF062 Secrete-Pair Gaussia Luciferase Assay Kit (1000 rxns) Detects Gaussia luciferase (GLuc) inquire

 

EndoFectin transfection reagents

Buy Cat.No. Product name Price Data sheets
EFM1004-01 EndoFectin™ Max transfection reagent (1 mL) $295 Download
EFM1004-02 EndoFectin™ Max transfection reagent (3 mL) $589
EFL1001-01 EndoFectin™ Lenti transfection reagent (1 mL) $245 Download
EFL1001-02 EndoFectin™ Lenti transfection reagent (3 mL) $489
EF005 EndoFectin™ HepG2 transfection reagent (1 mL) $355 Download
EF006 EndoFectin™ HepG2 transfection reagent (3 mL) $705
EF007 EndoFectin™ Expi293 transfection reagent (1 mL) $320  
EF008 EndoFectin™ Expi293 transfection reagent (3 mL) $640  

 

Validation Data

Figure 1. Activation of the GLuc-ON™ PR TRE by exogenous expression of PR. 293T cells were were co-transfected in the presence of 10 nM progesterone with (left to right) negative TRE clone + negative expression clone, negative TRE clone + PR expression clone, PR TRE clone + negative expression clone, or PR TRE clone + PR expression clone. Transfected cells were incubated for 40 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.