TCF/LEF transcriptional response element (TRE) clone

TCF/LEF transcriptional response element(TRE) clone for Wnt signaling pathway
TCF/LEF transcriptional response element(TRE) clone for Wnt signaling pathway
Transcriptional response element(TRE) negative control clone
Transcriptional response element(TRE) negative control clone
GAPDH positive control clone
GAPDH positive control clone
Secreted alkaline phosphatase (SEAP)<br />Expression clone
Secreted alkaline phosphatase (SEAP)
Expression clone

TCF/LEF transcriptional response element(TRE) clone for Wnt signaling pathway Transcriptional response element(TRE) negative control clone GAPDH positive control clone Secreted alkaline phosphatase (SEAP)
Expression clone

Price: $350.00 $350.00 $375.00 $395.00
Catalog#:
  • TR102
  • TR001
  • GAPDH-PG02
  • SEAP-PA01
Size: pEZX-PG02 pEZX-PG02 pEZX-PG02 pEZX-PA01
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Introduction

GeneCopoeia provides the GLuc-ON™ TCF/LEF transcriptional response element (TRE) clone for analysis of the effects on Wnt signaling pathways mediated by TCF/LEF transcription factors, which recruit β-catenin to activate the promoters of several genes critical for function of the Wnt pathway. Wnt signaling plays fundamental roles in embryonic development, and its misregulation has been implicated in diseases such as cancer and type 2 diabetes.

The GLuc-ON™ TCEF/LEF TRE clone contains tandem repeats of an TCF/LEF binding site upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The TCF/LEF response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ TCEF/LEF TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.