Introduction
GeneCopoeia’s GLuc-ON™ EGR1 transcriptional response element (TRE) clone enables analysis of the effects on genes regulated by early growth respionse protein 1 (EGR1), such as transforming growth factor-beta-1 (TGFB1) and fibronectin (FN1). EGR1 is a transcription factor that responds to mitogenic stimulation. EGR1 regulates many processes, such as neuron activity, wound healing, apopotosis, and angiogenesis, and is known to both suppress and promote tumor growth.
The GLuc-ON™ EGR1 TRE clone contains tandem repeats of an EGR1 binding site upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The EGR1 response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ EGR1 TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.
Figure 1. Activation of the GLuc-ON™ EGR1 TRE by treatment with phorbol 12-myristate 13-acetate (PMA). HEK293a cells were transfected with a negative control clone or the GLuc-ON™ EGR1 TRE clone. Transfected cells were incubated for 24 hours, then treated with different amounts of PMA for 24 hours. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as luminance fold activation.