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GeneHero™ CRISPRi Stable Cell Lines

GeneHero™ CRISPRi Stable Cell Lines are mammalian cell lines stably expressing dCas9-KRAB fusion protein for the study of transcriptional inhibition of endogenous genes. These CRISPRi cell lines have stably integrated human codon-optimized dCas9 fused to a transcriptional repressor peptide KRAB to silence target gene expression.

The cell lines are monoclonal with verified dCas9-KRAB genome integration and expression. Simply introduce promoter-targeting sgRNA or CRISPRi sgRNA libraries into the CRISPRi cell lines. They are ideal for the study of gene expression inhibition and genetic screening.

CRISPRi Transcription inhibition by CRISPRi cell line expressing dCas9-KRAS

Verification of CRISPRi Transcription Inhibition in GeneHero™ CRISPRi Cell Lines

CRISPR transcription inhibition in cell line SL372

As shown in the figures above, the transcription inhibition of DPH2 (A) was measured by qPCR in the HeLa cell line expressing dCas9-KRAB (Cat# SL372) with or without transcription inhibition. The amplification curves of CRISPRi cell lines transduced with sgRNA for the promoter of DPH2 (A) are shown in red; those without sgRNA transduction are shown in blue. The transcription level of DPH2 is normalized to that of GAPDH in the same cell line for comparison. The transcription fold change of the gene is shown (B) by comparison of cell line transduced with and without sgRNA.

 

 

Certificate of Analysis: GeneHero™ CRISPRi Stable Cell Line     Available upon request.

 

Buy Catalog# Cell Line Selection Marker Description Price, Academic Price, Industry
SL371 HEK293 Blasticidin HEK293 stably expressing dCas9-KRAB $945 $2695
SL372 HeLa Blasticidin HeLa stably expressing dCas9-KRAB $1395 $3995

 

Disclaimer: Pricing for premade cell lines are for customers in the US and Canada only. International customers, please contact your local distributor for pricing.

We also offer services for:

 

  • Custom CRISPRi Cell Lines
  • CRISPRi sgRNA

Contact us at inquiry@genecopoeia.com, or call 866-360-9531 or 301-762-0888 for a quote.