GeneHero™ CRISPRi Stable Cell Lines are mammalian cell lines stably expressing dCas9-KRAB fusion protein for the study of transcriptional inhibition of endogenous genes. These CRISPRi cell lines have stably integrated human codon-optimized dCas9 fused to a transcriptional repressor peptide KRAB to silence target gene expression.
The cell lines are monoclonal with verified dCas9-KRAB genome integration and expression. Simply introduce promoter-targeting sgRNA or CRISPRi sgRNA libraries into the CRISPRi cell lines. They are ideal for the study of gene expression inhibition and genetic screening.
Disclaimer: Pricing for premade cell lines are for customers in the US and Canada only. International customers, please contact your local distributor for pricing. To obtain the datasheet for each cell line, please click on the designated catalog number.
Verification of CRISPRi Transcription Inhibition in GeneHero™ CRISPRi Cell Lines
As shown in the figures above, the transcription inhibition of DPH2 (A) was measured by qPCR in the HeLa cell line expressing dCas9-KRAB (Cat# SL372) with or without transcription inhibition. The amplification curves of CRISPRi cell lines transduced with sgRNA for the promoter of DPH2 (A) are shown in red; those without sgRNA transduction are shown in blue. The transcription level of DPH2 is normalized to that of GAPDH in the same cell line for comparison. The transcription fold change of the gene is shown (B) by comparison of cell line transduced with and without sgRNA.