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ORF cDNA Clone Collections — Comprehensive Solutions for Gene Expression Studies

GeneCopoeia is the original manufacturer of human and mouse expression-ready, full-length ORF cDNA clones. With a comprehensive offering, GeneCopoeia has a specific ORF clone for every application and every budget.

Overexpression of genes and proteins is a valuable component of research in functional genomics, proteomics and systems biology. However, generation of expression-ready ORF clones often requires multi-step cloning processes, lengthy verification, and sequence analysis. GeneCopoeia provides a large variety of expression systems, promoters, fusion tags and selection markers. Researchers can request to either have their gene of interest cloned into a particular vector, or simply order from GeneCopoeia’s vast collection of premade expression-ready ORF clones. All ORFs are fully sequence-verified and ready-for-expression.

OmicsLink™ Expression-Ready ORF cDNA clones represent the largest collection (over 45,000) of expression-ready and sequence-verified human and mouse ORF clones. Learn More

OmicsLink™ Expression-Ready ORF cDNA clones are generated from sequence validated full-length cDNA clones or high-quality cDNA libraries. ORFs are constructed in expression vectors using GeneCopoeia’s proprietary RecJoin™ cloning technology. With a comprehensive selection of over 100 expression vectors and more than 50 single or tandem fusion tags, researchers have the flexibility to find the most suitable constructs for their functional genomics, proteomics, and system biology research. You can request to either have your gene-of-interest cloned into a chosen vector with unique features, or simply order from our vast collection of pre-made human and mouse expression-ready ORF clones.

GeneCopoeia guarantees that all full-length OmicsLink™ ORFs are free of PCR or cloning errors, frameshifts, premature stop codons, and single nucleotide polymorphisms (SNPs) not known to occur in nature.

ORFEXPRESS™ Gateway® PLUS ORF cDNA shuttle clones are fully sequence-verified Gateway® ORF entry clones. These clones are compatible with both recombination and traditional cloning using multiple cloning sites (MCS). Learn More

Transfer of an ORF from ORFEXPRESS™ cDNA shuttle clones to a destination vector is simple and rapid, and  frees you from tedious, time-consuming, multi-step sub-cloning processes involving enzyme digestions, ligations, gel electrophoresis and purification.

ORFeome Collaboration cDNA clones are third-party constructed and sequence-verified Gateway® ORF entry clones enabling transfer into Gateway® compatible destination vectors. Learn More

A “good value” based on our rating system, ORFeome clones were generated as a result of a collaboration between various research institutes including Dana Farber Cancer Institute-Center for Cancer Systems Biology, NCBI, WTSI/HAVANA group and the IMAGE Consortium. Due to variable quality, GeneCopoeia relies on the ORFeome collaboration clones’ Good Faith agreement, which we recommend you read.

ORF cDNA clone Selection Guide:

Option 1   BEST VALUE Option 2    HIGH VALUE Option 3 GOOD VALUE Option 4    THRIFTY
Products
OmicsLink™ Expression-Ready ORF cDNA Clones ORFEXPRESS™ Gateway® PLUS Shuttle Clones ORFeome Collaboration Clones in Gateway® Entry Vector GeneCopoeia Full-Length cDNA Clones
Primary differences
  • Ready to express; minimum work
  • Requires multiple subcloning steps
  • Requires multiple subcloning steps
  • Multiple cloning sites not present
  • Requires multiple subcloning steps
  • Includes 5’ and 3’ UTRs
Unique features
  • Fully sequenced
  • 100+ vectors
  • 50+ tags
  • Numerous promoters
  • ORF-only (no 5’ or 3’UTRs)
  • Multiple cloning site built in
  • Ribosomal binding sites included (Shine-Dalgarno and Kozak sequences)
  • ORF only (no 5’ or 3’ UTRs)
  • Kozak sequence built in
  • ORF only (no 5’ or 3’ UTRs)
  • Full-length, whole transcript cDNA templates with 5’ and 3’ UTRs
Advantages
  • Expression-ready
  • No subcloning necessary
  • Optimal translational context
  • Multiple applications
  • Less work; saves time
(Compared to regular Gateway®or ORFeome clones)                                  
  • Convenient for both recombination and traditional cloning
  • Optimal translational context
  • Can be used for recombination cloning
  • Low initial investment
Disadvantages

(Compared to OmicsLink Expression-Ready clones)
  • Requires several cloning steps
  • No promoter present (not expression-ready)
  • Purchase of destination or empty vector required
  • Requires transfer into destination or empty vector for expression
  • Requires validation
  • Extra cost and time required
(Compared to Gateway PLUS or Expression-Ready clones)
  • Requires several cloning steps
  • No promoter present(not expression-ready)
  • Not suitable for bacterial expression
  • Multiple cloning sites not present
  • Purchase of destination or empty vector required
  • Requires transfer into destination or empty vector for expression
  • Requires validation
  • Extra cost and time required
  • Requires multiple steps of cloning; labor intensive
  • No promoter present(not expression-ready)
  • Must purchase empty vector
  • Requires transfer intoempty vector for expression
  • Requires validation
  • Extra cost and time required

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Expression-ready ORF cDNA clones Cloning process from Gateway PLUS Shuttle ORF cDNA clones Cloning process from full-length whole transcript cDNA clones
   

Read more – OmicsLink™ ORF cDNA brochure