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Lenti-Pac Lentivirus Concentration Solution

Introduction

The Lenti-Pac™ Lentivirus Concentration Solution is designed for quick and simple concentration of lentiviral particles. The concentration is accomplished by mixing the proprietary reagent with the lentiviral supernatant, followed by a short incubation then centrifugation with an ordinary centrifuge.

 

Advantages

  • Recover up to 90% or more lentivirus
  • Increase titer (Transduction Units/ml) by 10X – 100X
  • Concentrate all types of lentiviral particles
  • Whole process can be finished in as short as ~ 3 hours
  • No ultracentrifugation is required

 

Figure 1. Concentration of lentiviral particles. Lentiviral particles expressing GFP (2×107 TU/ml) were concentrated 10 times using Lenti-Pac™ lentivirus concentration solution. The concentrated lentiviral particles were used to transduce H1299 cells in a 24-well plate. The pictures were taken 48 hours after the transduction. A: 0.2 µl of Standard lentivirus; B: 0.02 µl of concentrated lentivirus (incubated 4 hours with the concentration solution before centrifugation). C: 0.02 µl of concentrated lentivirus (incubated 16 hours with the concentration solution before centrifugation).