Introduction
GeneCopoeia’s Lenti-Pac™ HIV Expression Packaging Reagents are engineered for efficient, do-it-yourself lentiviral particle production. When combined with GeneCopoeia lentiviral constructs, the results are high viral titers and robust expression levels. We offer four Lenti-Pac™ Lentiviral Packaging Reagents, each available separately.
- Lenti-Pac™ HIV expression packaging kit. Comes with an optimized lentiviral packaging plasmid mix, eGFP positive control plasmid, EndoFectin™ Lenti, a transfection reagent optimized for virus production, and TiterBoost™ reagent, which increases titers 5-10 fold. This 3rd-generation packaging system ensures high-titer production of particles that are incapable of self-replication.
- Lenti-Pac™ 293Ta packaging cell line. Enables easy and efficient lentiviral plasmid transfection for high-level expression of viral proteins required for packaging.
- Lenti-Pac™ concentration solution. Designed for quick and simple concentration of lentiviral particles. Concentration is achieved by mixing the proprietary reagent with the lentiviral supernatant, followed by a short incubation and centrifugation.
- Lenti-Pac™ titration kit. Designed for quick and simple titration of lentiviral particles. Used for etermining viral copy number and to confirm the success of packaging reactions. Includes reagents for RNA extraction and qRT-PCR, as well as a standard control.
- Lenti-Pac™ Lentivirus Storage Buffer. Using this product to dissolve lentivirus particles can improve the stability of lentivirus at -80°C and effectively preserve functional viral particles during repetitive freeze-thaw cycles, effectively slowing down the decline in virus titer and preserving lentiviral infectivity. It is suitable for resuspending lentivirus after ultracentrifugation, polyethylene glycol precipitation (PEG precipitation), and ultrafiltration concentration.
Catalog# | Product | Description | User manual |
---|---|---|---|
LT001 | Lenti-Pac™ HIV Expression Packaging Kit(20 reactions) | HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent | Download Manual |
LT002 | Lenti-Pac™ HIV Expression Packaging Kit(40 reactions) | HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin Lenti Transfection Reagent | Download Manual |
LT005 | Lenti-Pac™ HIV qRT-PCR Titration Kit(20 RT reactions, 50 PCR reactions) | qRT-PCR based titration to determine the copy numbers of HIV lentiviral particles. | Download Manual |
LT006 | Lenti-Pac™ HIV qRT-PCR Titration Kit(40 RT reactions, 100 PCR reactions) | qRT-PCR based lentiviral titration to determine the copy numbers of HIV lentiviral particles. | Download Manual |
LT007 | Lenti-Pac™ Lentivirus Concentration Solution(50 ml) | Quick and simple concentration of lentiviral particles | Download Manual |
LT008 | Lenti-Pac™ 293Ta Cell Line(1.5 x 106 cells) | 293Ta Lentiviral packaging cell line | Download Manual |
LT039 | Lenti-Pac™ Lentivirus Storage Buffer | Dissolve lentivirus particles, effectively slowing down the decline in virus titer and preserving lentiviral infectivity. | Download Manual |
MP004 | Mycoplasma PCR detection kit | Quick detection of mycoplasma contamination in cell culture or virus | Download Manual |