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ExProfile™ Pathway Gene qPCR Arrays

For focused group profiling of pathway-related gene expression

Human Pathway qPCR Arrays

ExProfile™ Pathway Gene qPCR Arrays profile the expression of pathway-related genes, which are carefully chosen for their close pathway correlation based on a thorough literature search of peer-reviewed publications. In each 96-well catalog array plate there are up to 84 pairs of All-in-One™ qPCR primers, which have been pre-validated and deposited in designated wells. In each plate there also are 12 wells of controls for monitoring the efficiency of the entire experimental process: from reverse transcription to qPCR reaction.

 

Pathway Array List

Important! Select your PCR instrument to find matching arrays:

If your PCR instrument is not listed here, please contact us at 1-866-360-9531 or inquiry@genecopoeia.com

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Select matching PCR array products:

   
AMPK Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG004 ExProfile™ Human AMPK Signaling Related Gene qPCR Array Human 336 4 x 96-well Datasheet
Array layout
Primer list
Angiogenesis Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG002 ExProfile™ Human Angiogenesis Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Angiogenic Growth Factors & Angiogenesis Inhibitors Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG019 ExProfile™ Human Angiogenic Growth Factors & Angiogenesis Inhibitors Related Gene qPCR Array Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Antigen Processing & Presentation Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG003 ExProfile™ Human Antigen Processing & Presentation Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Apoptosis
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG099 ExProfile™ Human Apoptosis Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Autophagy Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG001 ExProfile™ Human Autophagy Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Breast Cancer & Estrogen Receptor Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG005 ExProfile™ Human Breast Cancer & Estrogen Receptor Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
cAMP/Ca2+ Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG009 ExProfile™ Human cAMP/Ca2+ Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Cancer Drug Resistance & Metabolism Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG007 ExProfile™ Human Cancer Drug Resistance & Metabolism Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Cell Cycle Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG006 ExProfile™ Human Cell Cycle Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Cell Cycle Toxicity and Cancer Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG053 ExProfile™ Human Cell Cycle Toxicity and Cancer Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Cytokine Receptor Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG008 ExProfile™ Human Cytokine Receptor Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Dendritic & Antigen Presenting Cell Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG010 ExProfile™ Human Dendritic & Antigen Presenting Cell Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
DNA Damage and Repair Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG012 ExProfile™ Human DNA Damage and Repair Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Drug Metabolism Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG011 ExProfile™ Human Drug Metabolism Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
EGF/PDGF Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG015 ExProfile™ Human EGF/PDGF Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Electron Transport Toxicology Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG014 ExProfile™ Human Electron Transport Toxicology Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Embryonic Stem Cells Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG016 ExProfile™ Human Embryonic Stem Cells Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Endothelial Cell Biology Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG017 ExProfile™ Human Endothelial Cell Biology Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Extracellular Matrix and Adhesion Molecules Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG013 ExProfile™ Human Extracellular Matrix and Adhesion Molecules Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
FoxP3 Target
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG018 ExProfile™ Human FoxP3 Target Genes qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
General Toxicology Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG020 ExProfile™ Human General Toxicology Related Gene qPCR Array Human 336 4 x 96-well Datasheet
Array layout
Primer list
Growth and Development Toxicity Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG021 ExProfile™ Human Growth and Development Toxicity Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Hedgehog Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG022 ExProfile™ Human Hedgehog Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Hematopoietic Stem Cells and Hematopoiesis Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG025 ExProfile™ Human Hematopoietic Stem Cells and Hematopoiesis Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
HIV Infection and Host Response Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG023 ExProfile™ Human HIV Infection and Host Response Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Hypoxia Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG026 ExProfile™ Human Hypoxia Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Immune Complement Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG033 ExProfile™ Human Immune Complement Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Immunology Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG031 ExProfile™ Human Immunology Related Gene qPCR Array Human 336 4 x 96-well Datasheet
Array layout
Primer list
Inflammatory Cytokines & Receptors Relayed
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG028 ExProfile™ Human Inflammatory Cytokines & Receptors Relayed Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Inflammatory Response and Autoimmunity Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG048 ExProfile™ Human Inflammatory Response and Autoimmunity Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Innate & Adaptive Immune Response Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG027 ExProfile™ Human Innate & Adaptive Immune Response Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Innate Immune Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG030 ExProfile™ Human Innate Immune Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Insulin Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG024 ExProfile™ Human Insulin Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Interferon Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG032 ExProfile™ Human Interferon Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Interferon Signaling & Response Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG029 ExProfile™ Human Interferon Signaling & Response Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
JAK/STAT Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG034 ExProfile™ Human JAK/STAT Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Lipoprotein Signaling & Cholesterol Metabolism Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG036 ExProfile™ Human Lipoprotein Signaling & Cholesterol Metabolism Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
MAP Kinase Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG038 ExProfile™ Human MAP Kinase Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Neurogenesis and Neural Stem Cell Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG042 ExProfile™ Human Neurogenesis and Neural Stem Cell Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Neuroscience Ion Channels & Transporters Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG039 ExProfile™ Human Neuroscience Ion Channels & Transporters Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Neurotransmitter Receptors and Regulators Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG044 ExProfile™ Human Neurotransmitter Receptors and Regulators Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
NFΚΒ Signaling Pathway Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG035 ExProfile™ Human NFΚΒ Signaling Pathway Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Nitric Oxide Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG041 ExProfile™ Human Nitric Oxide Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Notch Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG043 ExProfile™ Human Notch Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Nuclear Receptors and Coregulators Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG040 ExProfile™ Human Nuclear Receptors and Coregulators Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Osteogenesis Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG046 ExProfile™ Human Osteogenesis Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Oxidative Stress and Antioxidant DefenseRelated
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG045 ExProfile™ Human Oxidative Stress and Antioxidant DefenseRelated Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
PI3K-AKT Signaling Realted
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG047 ExProfile™ Human PI3K-AKT Signaling Realted Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Signal Transduction Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG051 ExProfile™ Human Signal Transduction Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Stem Cell Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG049 ExProfile™ Human Stem Cell Related Gene qPCR Array Human 420 5 x 96-well Datasheet
Array layout
Primer list
Stress & Toxicity Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG050 ExProfile™ Human Stress & Toxicity Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
T Cell Anergy & Immune Tolerance Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG057 ExProfile™ Human T Cell Anergy & Immune Tolerance Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
T helper 17 (Th17) Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG058 ExProfile™ Human T helper 17 (Th17) Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
T-cell and B-cell Activation Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG052 ExProfile™ Human T-cell and B-cell Activation Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Targets of Wnt/?-catenin Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG060 ExProfile™ Human Targets of Wnt/?-catenin Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
TGF-β Signaling Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG054 ExProfile™ Human TGF-β Signaling Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Th1-Th2-Th3 Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG055 ExProfile™ Human Th1-Th2-Th3 Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Tumor Metastasis Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG056 ExProfile™ Human Tumor Metastasis Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Tumor Necrosis Factor (TNF) Ligand and Receptor Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG037 ExProfile™ Human Tumor Necrosis Factor (TNF) Ligand and Receptor Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list
Ubiquitination (Ubiquitylation) Related
Buy Cat. No. Product name Species Number of genes Number of plates Document
QG059 ExProfile™ Human Ubiquitination (Ubiquitylation) Related Gene qPCR Array Human 84 1 x 96-well Datasheet
Array layout
Primer list

 

 

 

 

 

Select RT-PCR reagents

Buy Catalog# Product Description Price
QP008 All-in-One™ First-Strand cDNA Synthesis Kit for gene qPCR array (20 RT reactions) M-MLV RT (RNase H-), Reaction Buffer, RNase Inhibitor, dNTPs, Oligo(dT)18, Random Primer, dd H2O and spike-in control (for use with gene qPCR arrays) $129
QP009 All-in-One™ First-Strand cDNA Synthesis Kit for gene qPCR array (60 synthesis reactions) M-MLV RT (RNase H-), Reaction Buffer, RNase Inhibitor, dNTPs, Oligo(dT)18, Random Primer, dd H2O and spike-in control (for use with gene qPCR arrays) $285
QP001 All-in-One™ qPCR Mix (200 qPCR reactions) High-fidelity, hot-start DNA polymerase,ROX reference dye,optimized reaction buffer and dNTPs $169
QP002 All-in-One™ qPCR Mix (600 qPCR reactions) High-fidelity, hot-start DNA polymerase,ROX reference dye,optimized reaction buffer and dNTPs $389
QP004 All-in-One™ qPCR Mix (1000 qPCR reactions) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $635
QP005 All-in-One™ qPCR Mix (4000 qPCR reactions) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $1995
QP020 RNAzol® RT RNA Isolation Reagent Easy isolation of mRNA, microRNA or total RNA $69

 

 

 

 

 

 

 

How It Works

How qPCR Arrays Work

Gene qPCR array experimental work flow

Figure 1. Gene qPCR array experimental work flow.

 

Key advantages

Close pathway correlation

  • Genes are carefully chosen for their close pathway correlation based on a thorough literature search of peer-reviewed publications

Robust performance

  • Sensitive – Detects as low as 4 copies of RNA using ExProfile qPCR array and recommended reagents/conditions
  • Broad linearity – Simultaneously detects mRNAs at different expression levels
  • Reproducible – High reproducibility (R2> 0.99) for inter-array and intra-array replicates

Validated primers

  • Each primer is designed using a proprietary algorithm and has been experimentally validated

 

 

Custom Arrays and Related Products

Custom qPCR Arrays: Request a Quote

GeneCopoeia offers 96-well and 384-well custom array types and each array type has 6 layouts to choose from depending on the number of genes and replicates to be analyzed. Different controls can also be included in the arrays to help monitor the sample quality, RT and PCR reaction efficiencies and replicates reproducibility.

  • Genomic DNA control (GDC): detects genomic DNA contamination.
  • Spike-in reverse transcription control (RT): monitors the efficiency of the RT reaction.
  • Positive PCR control (PCR): verifies the PCR efficiency.
  • Housekeeping genes (HK): can be used as endogenous positive controls and for array normalization.

For the best performance, All-in-One™ First Strand cDNA Synthesis Kit and All-in-One™ qPCR Mix are the recommended and supported reagents for use with these arrays.

 

All-in-One qPCR validated primers 

The All-in-One™ qPCR human- mouse- or rat-specific primers are designed by a proprietary algorithm and validated for precision performance. Primer validation includes melting curve to ensure amplification of the correct target DNA (Figure 2). When used in combination with the All-in-One SYBR® Green qPCR Mix, the All-in-One primers deliver reliable and reproducible high performance in quantitative PCR assays.

 

Amplification curves
Melting curves Agarose Gel Electrophoresis Validation Result

Figure 2. Fourty-five pairs of gene-specific All-in-One™ qPCR primers were experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted genes. A cDNA pool, containing reverse transcribed products of total RNA from 10 different human tissue (lung, liver, testicle, ovary, spleen, brain, placenta, pancreas, heart and mammary), was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2X All-in-One qPCR Mix. Reactions were incubated for 10 min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15 sec. using Bio-Rad iQ5 Instrument. At the end of the last cycle, the temperature was increased from 72 to 95°C to produce a melting curve. Amplification curves, melting curves and agarose gel electrophoresis (validation result for positive in odd-lane and no template control (NTC) in even lane) shown for the 10 samples.

* Non-specific amplification and absence of primer-dimers are ensured when All-in-One validated PCR primers and PCR mix are used together.
† Primers validated automatically for human, mouse and rat. Inquire for validation of other species.

Data analysis tool

Analyze the qPCR result using GeneCopoeia's online Data Analysis System (free).

Related arrays

Related reagents