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Comprehensive solutions for miRNA studies — detect, express, validate or knockdown miRNA today

Find your miRNA Expressway to Discovery™ at GeneCopoeia with the largest collection of miRNA precursor, inhibitor and 3' UTR target validation constructs. In addition, GeneCopoeia has luciferase reporter assay kits, validated qPCR miRNA primers and qRT-PCR miRNA detection kits-the most comprehensive collection of functional analysis tools anywhere.

GeneCopoeia microRNA comprehensive analysis tools enable efficient and fast protocols for investigating miRNA functions in cell culture.

With the GeneCopoeia collection of miRNA functional analysis and detection tools

Conduct single gene or pathway analysis
Study gain-of-function or loss-of-function effects
Discover pathways, study diseases and uncover developmental stages
Identification and validation of diagnostic and therapeutic biomarkers and targets

Product		Expressway to Discovery*
miExpress™ Precursor miRNA Expression Clones	750 human 450 mouse 270 rat	Over-express miRNA for functional studies
miArrest™ miRNA Inhibitor Clones and Synthetic Oligonucleotides	750 human 450 mouse 270 rat	Knockdown miRNA for functional studies
miTarget™ miRNA 3’UTR Target Sequence Expression Clones	25,000 human 25,000 mouse	Do functional validation of predicted targets
Luc-Pair™ miR Luciferase Assays	Optimized for GeneCopoeia miRNA 3' UTR target validation constructs	Study miRNA-target gene interaction
OmicsLink™ Expression-Ready ORF cDNA Clones	20,000 human 15,000 mouse	Achieve efficient gain-of-function studies
All-in-One™ miRNA qRT-PCR Detection Kits	SYBR® Green-based for quantitative expression profiling	Accurately quantify miRNA expression
All-in-One™ miRNA qPCR Primer	Morethan 700 human, mouse and rat primers	Validated for robust, reproducible and reliable quantitation of miRNA activity
EndoFectin™ Transfection Reagents	Optimized for specific cell types	Transfect efficiently and achieve reliable and reproducible results

*Not sure which combination of products is right for your project? Contact us with a few details and we will provide recommendations especially designed for you.

miExpress Precursor miRNA Expression Clones

Over-express precursor miRNAs using lentiviral or non-viral vector based constructs with eGFP reporter genes.

Features of miRNA expression clones

Easier delivery into dividing, non-dividing and difficult-to-transfect target cells.
Stable cell-line selection marker (neomycin) enables regulation studies of both long-term and transient expression.
Vector-plasmid transfection and virus-infection transduction efficiencies are monitored with the eGFP reporter protein.
Fully-sequenced expression cassettes of all miRNA expression constructs.

Learn more about precursor miRNA expression clones.

Figure 1. Lentiviral vector based precursor miRNA expression clones and the mechanism of vector mediated miRNA gene regulation

miArrest miRNA Inhibitors

Achieve stable or transient knockdown of miRNAs using vector-based constructs or chemically synthesized single-stranded oligonucleotide.

Features of miRNA inhibitors

Lentiviral or non-viral backbone for dividing, non-dividing and difficult-to-transfect cells.
Simple screening of transfected or infection-transduced cells with reporter fluorescent protein.
Synthetic oligonucleotide chemically enhanced to improve longevity and efficiency.

Learn more about miRNA inhibitor clones and oligonucleotides.

mirna inhibitiors mechanism of action

Figure 2. Simplified mechanism of action for vector-based miRNA inhibitors

miTarget miRNA Target Sequence 3' UTR Expression Clones

Conduct functional validation of predicted targets using genome-wide microRNA 3'UTR target constructs available in mammalian expression vector systems with single and dual luciferase reporter genes.

Features of microRNA 3' UTR target clones

Expression plasmids transcribe a chimeric mRNA consisting of the firefly or Gaussia luciferase coding sequence and a 3' UTR target sequence.
The Renilla luciferase or alkaline phosphatase genes can be used as a tracking indicator for successful transfection and expression of the miRNA constructs in target cells.
The regulatory effect of a particular miRNA on its potential target is assessed with a functional assay for the firefly or Gaussia luciferase.

Learn more about miRNA 3' UTR target expression clones.

mirna 3’ UTR target vector backbones

Figure 3. Vector features for human miRNA target sequence expression clones

Luc-Pair miR Luciferase Assay Kit

Study miRNA-target interaction using the Luc-Pair miR Luciferase Assay Kit available in a convenient 96-well plate format for measuring firefly and Renilla luciferases sequentially. Optimized for use with GeneCopoeia miRNA 3' UTR Target Sequence Expression Clones, Luc-Pair miR assays enable easy, cost effective validation of miRNA activity.

Advantages of Luc-Pair miR Luciferase Assay

High sensitivity in dynamic range
Dual reporter system
Reproducible
Cost effectiveness
Simple assay format
Suitable for high-throughput screening

Learn more about miR luciferase assay kit.

Data showing the inhibitory effect of miRNA on a 3' UTR target sequence expression clone

Figure 4. The inhibitory effect of miRNA on a target sequence (3' UTR) expression clone can be measured with GeneCopoeia Luc-Pair miR Luciferase Assay kit. HEK 293 cells were plated on a 6-well plate. On the second day, the cells were transfected with 1.0 µg of Lin28 3' UTR miRNA target sequence expression clone in pEZX-MT01 vector with fLuc (Product ID: HmiT019205) and 1.4 µg of miR-125a precursor miRNA expression clone in pEZX-MR03 vector with eGFP (Product ID: HmiR0309) and miRNA control clone as indicated in the figure. The cells were transferred to a 96-well plate 18 hours after transfection and cultured for another 24 hours. Both firefly luciferase and Renilla luciferase activities were measured and data was recorded on Victor II machine. Firefly luciferase activity was then normalized with Renilla luciferase activities in the same well.