Resources

CRISPR-Cas9 Specificity: Taming Off-target Mutagenesis

  Ed Davis, Ph.D. Abstract Genome Editing-the ability to make specific changes at targeted genomic sites in complex organisms-is of fundamental importance in biology and medicine (Bogdanove & Voytas, 2011; van der Oost, et al.,2013). Recently, the CRISPR...
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Genome Editing Technology From GeneCopoeia, Inc.

Ed Davis, Ph.D. Abstract Genome Editing-the ability to make specific changes at targeted genomic sites-is fundamentally important to researchers in biology and medicine (Bogdanove & Voytas, 2011; van der Oost, et al., 2013). Two genome editing technologies have...
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Knockout by TALEN or CRISPR vs. Knockdown by shRNA or siRNA

  Ed Davis, Ph.D. Recent advances in technologies for genome editing-the use of TALEN or CRISPR to make targeted, permanent changes to genes-have revolutionized molecular genetics. They have also presented users with a choice between these relatively new...
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GeneCopoeia Genome Editing Tools for Safe Harbor Integration in Mice and Humans

  Ed Davis, Liuqing Qian, Ruiqing lI, Junsheng Zhou, and Jinkuo Zhang Introduction The ability to introduce transgenes into biological systems, such as cultured cells or animals, is of fundamental importance in biology and medicine. Applications for the...
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ORF clones: many tags, many decisions

  Ed Davis, Ph.D. Introduction Fusion tagging-the practice of adding amino acids in-frame to one end of a native protein-is a well- established strategy for many applications, including protein...

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