Recombination cloning technology is based on the site specific recombination reaction of phage lambda, and is the same principle as Gateway® technology, an efficient biochemical process that conserves genetic information (see Figure 1). The transmission of an ORF from EZShuttle Gateway® PLUS ORF Clones to a pEZ™ or Gateway® Destination Vector is a one step simple and rapid operation (60 minutes at room temperature), which saves a significant amount of time and effort from the tedious, multiple step, sub-cloning process involving enzyme digestions, ligations, gel electrophoresis and purification (see Figure 2). Furthermore, GeneCopoeia provides a family of pEZ™ vectors that contain different promoters and fusion-tags that meet the distinct requirements for production and purification of desired recombinant proteins in several expression hosts.
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| Figure 1. Site specific recombination reaction of phage lambda, Click on the image to see enlarged image | Figure 2. One step transmission of an ORF from EZShuttle™ Gateway PLUS ORF clones to a pEZ™ or Gateway destination vector. Click on the image to see enlarged image |
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Note: Gateway® is a registered trademark of Life Technologies..