GeneCopoeia’s GLuc-ON™ EGR1 transcriptional response element (TRE) lentiviral clone enables analysis of the effects on genes regulated by early growth response protein 1 (EGR1), such as transforming growth factor-beta-1 (TGFB1) and fibronectin (FN1). EGR1 is a transcription factor that responds to mitogenic stimulation. EGR1 regulates many processes, such as neuron activity, wound healing, apoptosis, and angiogenesis, and is known to both suppress and promote tumor growth.
The GLuc-ON™ PKC EGR1 lentiviral clone contains tandem repeats of EGR1 upstream of a minimal promoter and a secreted Gaussia luciferase reporter gene, allowing for robust and sensitive analysis without cell lysis. The EGR1 response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ EGR1 TRE lentiviral clone is provided as transfection-ready DNA. A negative (non-activating) control lentiviral clone is also available for purchase.
Validation Data
Figure 1. Activation of the GLuc-ON™ EGR1 TRE by phorbol 12-myristate 13-acetate (PMA). HEK293a cells were transduced with negative control lentiviral particles or the GLuc-ON™ EGR1 TRE lentiviral particles. Transduced cells were incubated for 48 hours, followed by 6 days of incubation in the presence of puromycin. Puromycin-resistant cells were then treated with PMA for 18 hours. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as the fold activation of luminescence.
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