Resources

Lentivirus: That’s My MOI, and I’m Sticking To It

  Ed Davis, Ph.D. Introduction Lentiviral vectors have proven invaluable for introducing genetic material into mammalian cells, either in culture or whole animals (reviewed in Mátrai, et al., 2010). Lentiviruses, primarily derived from human or feline...
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ORF clones: many tags, many decisions

  Ed Davis, Ph.D. Introduction Fusion tagging-the practice of adding amino acids in-frame to one end of a native protein-is a well- established strategy for many applications, including protein purification, immunoprecipitation, Western blotting, and in vivo...
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Genome Editing: Which Should I Choose, TALEN or CRISPR?

  Ed Davis, Ph.D. Introduction Genome Editing-the ability to make specific changes at targeted genomic sites-is of fundamental importance to researchers in biology and medicine (Bogdanove & Voytas, 2011; van der Oost, et al.,2013). Two genome editing...
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Genome Editing in Mammalian Cells: What Do I Do Next?

  Ed Davis, Ph.D. Genome Editing-the ability to make specific changes at targeted genomic sites-is of fundamental importance in biology and medicine (for reviews, see Bogdanove & Voytas, 2011; van der Oost, et al.,2013). Two genome editing technologies have...
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