GeneCopoeia provides the GLuc-ON™ TCF/LEF transcriptional response element (TRE) lentiviral clone for analysis of the effects on Wnt signaling pathways mediated by TCF/LEF transcription factors, which recruit β-catenin to activate the promoters of several genes critical for function of the Wnt pathway. Wnt signaling plays fundamental roles in embryonic development, and its misregulation has been implicated in diseases such as cancer and type 2 diabetes.
The GLuc-ON™ TCF/LEF TRE lentiviral clone contains tandem repeats of TCF/LEF upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The TCF/LEF response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ TCF/LEF TRE lentiviral clone is provided as transfection-ready DNA. A negative (non-activating) control lentiviral clone is also available for purchase.
Validation Data
Figure 1. Activation of the GLuc-ON™ Wnt TRE by lithium chloride. HEK293a cells were transduced with negative control lentiviral particles or the GLuc-ON™ TCF/LEF TRE lentiviral particles. Transduced cells were incubated for 48 hours, followed by 6 days of incubation in the presence of puromycin. Puromycin-resistant cells were then treated with lithium chloride for 18 hours. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.