PR transcriptional response element (TRE) Clone

PR transcriptional response element (TRE) clone for progesterone receptors signaling pathway
PR transcriptional response element (TRE) clone for progesterone receptors signaling pathway
Secreted alkaline phosphatase (SEAP)<br />Expression clone
Secreted alkaline phosphatase (SEAP)
Expression clone
Transcriptional response element(TRE) negative control clone
Transcriptional response element(TRE) negative control clone
GAPDH positive control clone
GAPDH positive control clone

PR transcriptional response element (TRE) clone for progesterone receptors signaling pathway Secreted alkaline phosphatase (SEAP)
Expression clone
Transcriptional response element(TRE) negative control clone GAPDH positive control clone

Price: $364.00 $411.00 $364.00 $390.00
Catalog#:
  • TR113
  • SEAP-PA01
  • TR001
  • GAPDH-PG02
Size: pEZX-PG02 pEZX-PA01 pEZX-PG02 pEZX-PG02
Qty:
Manual: DownloadDownloadDownload
View your cart


Introduction

GeneCopoeia’s GLuc-ON™ PR transcriptional response element (TRE) clone enables analysis of the activity of signal transduction mediated by progesterone receptors (PR). Progesterone receptors are transcription factors that, in response to stimulation by progesterone, lead to the proliferation of breast tissue. Aberrant function and/or expression of PRs is an important factor in the development of breast cancer

The GLuc-ON™ PR TRE clone contains tandem repeats of PR upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The PR response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ PR TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activated), and normalization (secreted alkaline phosphatase) control clones are also available for purchase.

User Manual

Validation Data

Figure 1. Activation of the GLuc-ON™ PR TRE by exogenous expression of PR. 293T cells were were co-transfected in the presence of 10 nM progesterone with (left to right) negative TRE clone + negative expression clone, negative TRE clone + PR expression clone, PR TRE clone + negative expression clone, or PR TRE clone + PR expression clone. Transfected cells were incubated for 40 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.