MEF2 transcriptional response element (TRE) clone

MEF2 transcriptional response element(TRE) clone for MEF2 signaling pathway
MEF2 transcriptional response element(TRE) clone for MEF2 signaling pathway
Transcriptional response element(TRE) negative control clone
Transcriptional response element(TRE) negative control clone
GAPDH positive control clone
GAPDH positive control clone
Secreted alkaline phosphatase (SEAP)<br />Expression clone
Secreted alkaline phosphatase (SEAP)
Expression clone

MEF2 transcriptional response element(TRE) clone for MEF2 signaling pathway Transcriptional response element(TRE) negative control clone GAPDH positive control clone Secreted alkaline phosphatase (SEAP)
Expression clone

Price: $364.00 $364.00 $390.00 $411.00
Catalog#:
  • TR109
  • TR001
  • GAPDH-PG02
  • SEAP-PA01
Size: pEZX-PG02 pEZX-PG02 pEZX-PG02 pEZX-PA01
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Introduction GeneCopoeia’s GLuc-ON™ MEF2 transcriptional response element (TRE) clone enables analysis of pathways that act through the myocyte enhancer factor 2 (MEF2) proteins. MEF2 is a family of 4 transcription factors (MEF2A, MEF2B, MEF2C, and MEF2D) in mammals that regulate genes involved in muscle development and cardiac stress. The GLuc-ON™ MEF2 TRE clone contains tandem repeats of an MEF2 binding site upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The MEF2 response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ MEF2 TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreted alkaline phosphatase) control clones are also available for purchase.
User Manual
 

Validation Data

    Figure 1. Activation of the GLuc-ON™ MEF2 TRE by treatment with phorbol 12-myristate 13-acetate (PMA). HEK293a cells were infected with Adenovirus particles expressing GFP and/or MEF2 clone, then treated with PMA for 24 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminance fold activation.