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CRISPR Insertion or Deletion Detection System

GeneCopoeia's IndelCheck™ CRISPR insertion or deletion (indel) detection system provides a powerful means for assisting you in your genome editing applications. The IndelCheck™ system can be used for:

  • CRISPR sgRNA functional validation (Figure 1) before undertaking long genome editing projects (3-6 months for genome edited cell lines, or 6-9 months for genome-edited mouse lines), saving you a great deal of time and effort by eliminating CRISPR sgRNAs with poor cleavage efficiency.
  • Screening cell clones for knockout (KO) and knock-in (KI) modifications (Figure 2).

Advantages

  • Complete system to simplify your CRISPR validation and edited clone screening
  • Robust amplification for the target site PCR. No genomic DNA isolation is required
  • Easy to use T7 endonuclease I assay with optimized conditions and positive control

Order Information

Order Information

The IndelCheck™ system consists of the following major components:

  1. Target site PCR kit V2.0. For amplification of targeted genomic regions from cell lysates without genomic DNA isolation. Now in Version 2.0, the Target Site PCR kit is an contains buffer, SuperHeRo™ DNA polymerase, and nucleotides together in one mix for greater convenience.
  2. T7 endonuclease I assay kit. For detection of CRISPR-introduced indel mutations near target site(s).
  3. Smart-Join™ Blunt-end PCR Cloning Kit​. For cloning of PCR products to be used for sequencing and identification of CRISPR-mediated genomic modifications.

 

 

 

IndelCheck™ CRISPR insertion or deletion (indel) detection system (version 2.0)

Buy Catalog # Product Description Price
IC001 IndelCheck™ CRISPR insertion or deletion detection system (50 rxns) Includes target site PCR kit (IC003) and T7 endonuclease I assay kit (IC005) $206
IC002 IndelCheck™ CRISPR insertion or deletion detection system (200 rxns) Includes target site PCR kit (IC004) and T7 endonuclease I assay kit (IC006) $622
IC003 Target site PCR Kit (version 2.0) , 50 rxns* PCR reagents for amplifying region flanking CRISPR target site, prior to T7 Endonuclease I digestion* $159
IC004 Target site PCR Kit (version 2.0), 200 rxns* PCR reagents for amplifying region flanking CRISPR target site, prior to T7 Endonuclease I digestion* $479
IC005 T7 endonuclease I assay kit, 50 rxns Cleave mismatched PCR products using T7 endonuclease I to detect indel mutations $99
IC006 T7 endonuclease I assay kit, 200 rxns Cleave mismatched PCR products using T7 endonuclease I to detect indel mutations $299
IC007 Smart-Join™ Blunt-end PCR Cloning Kit, 20 rxns Clone PCR products for sequencing and identification of CRISPR-mediated genomic modifications $249
IC008 Smart-Join™ Blunt-end PCR Cloning Kit, 100 rxns Clone PCR products for sequencing and identification of CRISPR-mediated genomic modifications $699

*Version 2.0 now contains buffer, SuperHeRo™ DNA polymerase, and nucleotides together in one mix for greater convenience compared with Version 1.0.