GeneCopoeia’s AAVPrime™ Adeno-associated virus (AAV) products are the ideal tools for expressing or modifying genes in a broad range of cell and tissue types, especially in vivo, with high efficiency and enhanced safety. GeneCopoeia’s optimized helper-free human AAV system allows viral packaging without potentially pathogenic helper adenovirus. Many pre-made AAV are available in 12 different serotypes, such as fluorescent reporters. You can also request us to produce custom AAV for genes up to 3 kb in length.
- High titers. Titer of purified AAV particles can be up to 1014 GC/ml.
- High adaptability. Virtually all serotypes are available.
- Versatile. Usable in a broad range of host cell and tissue types.
- Low toxicity. Does not integrate into the host genome.
- Low immunogenicity. Minimal host immune response.
- Safe. Not associated with any human disease
We offer AAV of virtually all serotypes with titers starting from 5×1012 GC/ml packaged with ORF cDNA, CRISPR/Cas9 or shRNA. Click the links below to search for your gene(s) of interest.
For custom AAV packaging service, please contact us at inquiry@genecopoeia.com to get a quote.
Choose from our collection of AAV vectors with different promoters and fluorescent markers for your gene expression. The AAV can be packaged with ORF cDNA clones up to 3 kb in length for long-term expression.
AAV Vector List
Vector name | Promoter | Promoter description | Reporter gene | Max length of insertion |
---|---|---|---|---|
pEZ-AV01 | CMV | Strong promoter, broad scope of application. | N/A | 2.4 kb |
pEZ-AV02 | EF-1α | Broad scope of application. Better choice than CMV for in vivo applications. | N/A | 3.2 kb |
pEZ-AV04 | CAG | Ubiquitous. Better choice than CMV for in vivo applications. | N/A | 2 kb |
pEZ-AV06 | CBh | Hybrid chicken beta actin promoter. Provides better long-term expression than CMV in neural cells. | N/A | 2.8 kb |
pEZ-AV07 | CMV | Strong promoter, broad scope of application. | IRES-eGFP | 1.3 kb |
pEZ-AV08 | EF-1α | Broad scope of application. Better choice than CMV for in vivo applications. | IRES-eGFP | 2 kb |
pEZ-AV09 | CAG | Ubiquitous. Better choice than CMV for in vivo applications. | IRES-eGFP | 0.9 kb |
pEZ-AV11 | CBh | Hybrid chicken beta actin promoter. Provides better long-term expression than CMV in neural cells. | IRES-eGFP | 1.7 kb |
pEZ-AV12 | CAMKlla | Highly-specific expression in excitatory neurons in the neocortex and hippocampus. | N/A | 2.4 kb |
pEZ-AV13 | hSYN | Human synapsin-1 gene promoter, neuronal-specific. | N/A | 3.2 kb |
pEZ-AV15 | MHC | Muscle-specific human promoter. | N/A | 3.3 kb |
pEZ-AV18 | MCK | Cardiac muscle-specific human promoter. | N/A | 3.0 kb |
pEZ-AV24 | MBP | Oligodendrocyte-specific expression in central nervous system. | N/A | 3.0 kb |
pEZ-AV33 | ALB | Liver-specific human promoter. | N/A | 2.0 kb |
We offer AAV packaged with
- SaCas9 and sgRNA in all-in-one vector
- sgRNA only (for SpCas9 or SaCas9)
- SpCas9 or SaCas9 only coming soon
AAV CRISPR Vectors
Vector name | CRISPR | Description | Reporter gene |
---|---|---|---|
pCRISPR-AD01 | SaCas9 & sgRNA | All-in-one AAV vector with SaCas9 and sgRNA | N/A |
pCRISPR-AV20 | sgRNA only | AAV vector expressing sgRNA driven by U6 promoter | N/A |
Vector | Promoter | Selection marker | Reporter Gene | Viral type |
---|---|---|---|---|
psi-AV03 | U6 | N/A | N/A | AAV |
psi-AVE001 | CMV | N/A | eGFP | AAV |
psi-AVE002 | CMV | N/A | mCherry | AAV |
psi-AVE003 | EF1a | N/A | eGFP | AAV |
psi-AVE004 | EF1a | N/A | mCherry | AAV |
Request a quote for custom pol III or pol II promoter-driven shRNA AAV services.
miRNA Precursor AAV particles |
Vector | Tracking gene | Selection marker | Promoter | Type |
---|---|---|---|---|
pEZX-MR14 | eGFP | Puromycin | CMV | AAV |
pEZX-MR15 | mCherry | Puromycin | CMV | AAV |
miRNA Inhibitor AAV particles |
Vector | Tracking gene | Selection marker | Promoter | Type |
---|---|---|---|---|
pEZX-AM06 | mCherry | Hygromycin | H1 | AAV |
pEZX-AM07 | mCherry | Hygromycin | U6 | AAV |
AAV Control Particles
Order AAV particles for controls and popular genes
AAV Technology Overview
Custom AAV Production with the AAVPrimeTM System
AAVPrime™ uses a helper virus-free system for the safe preparation of high titer AAV. HEK293T cells are co-transfected with three plasmids encoding the factors necessary for recombinant AAV packaging (Right Figure). The AAV particles are then either membrane purified or purified by two-phase partitioning for high-titer AAV ready for in vivo animal use.The destination vector carries your gene of interest (GOI) and two ITRs. You can choose from a variety of destination vectors carrying promoters including CMV, EF1a, CAG, CBh and other tissue-specific promoters, and reporter gene GFP for optimal gene expression and detection. (Learn more about our vector collection); the plasmid that carries the Rep and Cap genes from wild type AAV and the plasmid that carries adenovirus VA, E4 and E2A genes required for efficient AAV production are co-transfected with the destination vector for AAV packaging.
AAV Serotype Choices
GeneCopoeia provides AAV for all the commonly-occurring AAV serotypes, including AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV-DJ and AAV-DJ/8. The intensity of gene expression and tissue tropism depends highly on the AAV serotype used in different tissues.
Different serotypes differ mainly in surface protein (capsid). Carefully choosing the serotype enables robust gene expression in specific tissues with minimal immune response. Refer to the table below to compare the essential differences between different serotypes.
AAV Serotypes Available
Serotype | Primary Target Tissues | Description |
---|---|---|
AAV-1 | Muscle | Best for cardiac muscle, skeletal muscle, neuronal and glial tissue. |
AAV-2 | Muscle, Liver, Retina | Oldest and most commonly-used serotype. Best for neurons, muscle, liver, and brain. |
AAV-3 | Megakaryocytes | Best for megakaryocytes, muscle, liver, lung, and retina. |
AAV-4 | Retina | Best for neurons, muscle, brain, and retina. |
AAV-5 | Lung | Best for lung, neurons, synovial joint, retina, and pancreas. |
AAV-6 | Muscle, Lung | Best for lung, liver, and heart. |
AAV-7 | Muscle, Retina, Neurons | Best for muscle, neurons, and liver. |
AAV-8 | Liver | Best for muscle, brain, liver, and retina. |
AAV-9 | Various | Best for muscle, heart, liver, lung, and brain. |
AAV-10 | Pleura, CNS | Cloned from Cynomolgus, almost identical with AAVrh10 except for 12 amino acids in VP1. Best for lung, muscle, heart, CNS, and liver. |
AAV-DJ | Various | A mixture of 8 naturally-occurring serotypes. Efficiently transduces a wide variety of cell types in vitro. |
AAV-DJ/8 | Various | A variant of AAV-DJ with a mutation that permits infection of liver as well as other tissues in vivo. |
Technical Notes and MSDS
MSDS
Material Safety Data Sheet for AAVPrme™ AAV Particles
Technical Notes
Choosing Between Lentivirus and Adeno-associated Virus For DNA Delivery
Publications
View selected publications citing GeneCopoeia’s AAV products and services from the recent literature
2023- Doron, G., et al. (2023). Poly(ethylene glycol)-Based Hydrogel Microcarriers Alter Secretory Activity of Genetically Modified Mesenchymal Stromal Cells. ACS Biomater Sci Eng. doi: 10.1021/acsbiomaterials.3c00954 [AAVPrime™ AAV particles expressing IL1RN]
- Hasan, K.M., et al. (2023). Fatty Acid Excess Dysregulates CARF to Initiate the Development of Hepatic Steatosis. Cells doi: 10.3390/cells12071069 [AAVPrime™ AAV particles expressing CARF and AAV-empty vector; OMICSLINK™ CARF Lentiviral ORF expression clone]
- Hasan, M.N., et al. (2023). Combining ASBT inhibitor and FGF15 treatments enhances therapeutic efficacy against cholangiopathy in female but not male Cyp2c70 KO mice. J Lipid Res doi: 10.1016/j.jlr.2023.100340 [OMICSLINK™ Fgf15 ORF expression clone; AAVPrime™ AAV particles expressing Fgf15]
- Gannon, A-L., et al. (2022). A Novel Model Using AAV9-Cre to Knockout Adult Leydig Cell Gene Expression Reveals a Physiological Role of Glucocorticoid Receptor Signalling in Leydig Cell Function Int J Mol Sci doi: 10.3390/ijms232315015 [AAVPrime™ AAV particles expressing CRE and GFP]
- Chen, X., et al. (2022). Pitx3 deficiency promotes age-dependent alterations in striatal medium spiny neurons. Front Aging Neurosci doi: 10.3389/fnagi.2022.960479 [AAV-hSyn1-eGFP]
- Hogan, T.B., et al. (2022). Caveolin-1 Peptide Regulates p53-microRNA-34a Feedback in Fibrotic Lung Fibroblasts. iScience doi: 10.1016/j.isci.2022.104022 [AAVPrime™ AAV particles expressing microRNA inhibitors]
- Yang, B., et al. (2022). Evaluation of Early Biomarkers of Atherosclerosis Associated with Polychlorinated Biphenyl Exposure: An in Vitro and in Vivo Study. Environmental Health Perspectives doi: 10.1289/EHP9833 [AAVPrime™ AAV particles expressing HDAC7]
- Xia, Y., et al. (20220. Neuronal C/EBP/AEP pathway shortens life span via selective GABAnergic neuronal degeneration by FOXO repression. Science Advances doi: 10.1126/sciadv.abj8658 [AAVPrime™ AAV particles expressing FOXO1, shRNA targeting FOXO1]
- Song, M-Y., et al. (2022. Sirt6 reprograms myofibers to oxidative type through CREB-dependent Sox6 suppression. Nature Communications doi: 10.1038/s41467-022-29472-5 [AAVPrime™ AAV particles expressing Sirt6, shRNA targeting Sirt6]
- Lio, J., et al. (2022). C/EBPβ/AEP signaling couples atherosclerosis to the pathogenesis of Alzheimer’s disease. Molecular Psychiatry doi: 10.1038/s41380-022-01556-0 [AAVPrime™ AAV particles expressing shRNA targeting ApoE]
- Toan, N.K., et al. (2021). Choline Acetyltransferase Induces the Functional Regeneration of the Salivary Gland in Aging SAMP1/Kl -/- Mice. International Journal of Molecular Sciences doi: 10.3390/ijms22010404 [AAVPrime™ AAV particles expressing shRNA targeting ChAT]
- Zhang, W-B., et al. (2021). Activation of Nrf2 by miR-152 Inhibits Doxorubicin-InducedCardiotoxicity via Attenuation of Oxidative Stress, Inflammation,and Apoptosis. Oxidative Medicine and Cellular Longevity doi: 10.1155/2021/8860883 [AAVPrime™ AAV particles expressing mir-152 miRNA; AAVPrime™ AAV particles expressing shRNA targeting Nrf2]
- Kang, S.S., et al. (2021). ApoE4 inhibition of VMAT2 in the locus coeruleus exacerbates Tau pathology in Alzheimer’s disease. Acta Neuropathologica doi: 10.1007/s00401-021-02315-1 [AAVPrime™ AAV particles expressing ApoE4]
- Quan, D.N. and Shiloach, J. (2021). rAAV production and titration at the microscale for high- throughput screening. Human Gene Therapy doi: 10.1089/hum.2021.080 [AAVPrime™ AAV particles expressing EGFP]
- Lan, T., et al. (2021). MicroRNA-204-5p reduction in rat hippocampus contributes to stress-induced pathology via targeting RGS12 signaling pathway. Journal of Neuroinflammation doi: 10.1186/s12974-021-02299-5 [AAVPrime™ AAV particles expressing miR-204-5p]
- Chen, Y-T., et al. (2021). Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance. Nature Communications doi: 10.1038/s41467-021-27171-1 [AAVPrime™ AAV particles]
- Han, L., et al. (2020). Homocysteine-induced electrical remodeling via the mediation of IP3R1/Nav1.5 signaling pathway. Am J Transl Res. 2020; 12(7): 3822–3841 [AAVPrime™ AAV particles expressing shRNA targeting IP3R1]
- Luo, Y., et al. (2020). Upregulation of circ_0000199 in circulating exosomes is associated with survival outcome in OSCC. Scientific Reports doi: 10.1038/s41598-020-70747-y [AAVPrime™ AAV particles expressing shRNA targeting circ_0000199]
- Luo, Y., et al. (2020). A novel epigenetic regulation of circFoxp1 on Foxp1 in colon cancer cells. Cell Death & Disease doi: 10.1038/s41419-020-03007-6 [AAVPrime™ AAV particles expressing shRNA targeting circ_FoxP1]
- Millett, P.J., et al. (2020). Gene Editing to Impove Joint Function. United States Patent Applicaitons doi: 20200360454K [AAVPrime™ AAV particles expressing GFP]
- Zhao, Y. and Ren, J. (2019). MODIFIED IMMUNE CELLS HAVING ENHANCED FUNCTION AND METHODS FOR SCREENING FOR SAME. United States Patent Application 20190345491 [AAVPrime™ AAV particles expressing DNMT3A]