Home » FAQs » I would like to transfer the ORFs from ORFEXPRESS™ Gateway® Shuttle Clones into my own vectors that have specific features, but these vectors are not Gateway® compatible. How to do it?

I would like to transfer the ORFs from ORFEXPRESS™ Gateway® Shuttle Clones into my own vectors that have specific features, but these vectors are not Gateway® compatible. How to do it?

Answer: Each ORFEXPRESS™ Shuttle Clone has two multiple cloning sites (MCS) flanking the ORF. By choosing suitable restriction enzymes, you can cut out the entire ORF from ORFEXPRESS™ Shuttle clones and insert the ORF into your vectors by classical sub-cloning methods.