Luciferase Assays Why is it advantageous to use a secreted reporter over Firefly or Renilla luciferases? Answer: To visualize Firefly and Renilla luciferase activity, it is necessary to first...
FAQs
Protein expression
What kind of tag can I choose for protein expression? Answer: You can choose any desired tag for your target protein, as well as decide whether you want the tag to be placed at the N- or C-terminus....
qPCR arrays and reagents
What can GeneCopoeia's miProfile™ miRNA qPCR arrays and ExProfile™ gene qPCR arrays do for you? Answer: With these arrays, you don't need to spend time on primer design and validation...
Product FAQs
ORF cDNA clones shRNA clone sets Genome editing products (Genome-TALER™ TALEN and TALE-TF custom...
Luciferase Assays
Luciferase Assays Why is it advantageous to use a secreted reporter over Firefly or Renilla luciferases?...
Protein expression
What kind of tag can I choose for protein expression? Answer: You can choose any desired tag for your target protein,...
qPCR arrays and reagents
What can GeneCopoeia's miProfile™ miRNA qPCR arrays and ExProfile™ gene qPCR arrays do for you?...
Stable Cell Line Services
What types of cell-based services do you offer? Answer: There are two types of related services we currently offer in...
Genome editing products
General How do I order TALEN or CRISPR reagents from GeneCopoeia? Answer: If you are doing simple gene knockouts in...
shRNA clone sets
How do I order shRNA clones from GeneCopoeia? Answer: All you need to do is go to the shRNA clones search page, search...
Lentivirus
How do I order custom lentiviral particles from GeneCopoeia? Answer: The best way is to search for what you need on...
Products FAQs – ORF cDNA clones
ORF clones How do I order ORF clones from GeneCopoeia? Answer: All you need to do is go to the ORF clones search...
Product FAQs
ORF cDNA clones shRNA clone sets Genome editing products (Genome-TALER™ TALEN and TALE-TF custom services,...
What type of expression vectors are suitable for the transfer of the ORFs in ORFEXPRESS™ Gateway® Shuttle Clones we are interested in?
Answer: There is a suite of pDEST expression vectors (destination vectors) available from Invitrogen Corporation that...
Do you have ORFs from other species?
Answer: Currently, we only offer pre-made Human and Mouse ORF clones. We are in the process of producing full length...
I have done expression experiment, but there is no protein. Why?
Answer: A correct DNA sequence with a promoter does not guarantee you to get protein expressed. Many factors...
Have you done expression test of each gene?
Answer: All expression clones in pReceiever-B01 and pReceiver-WG02/WG03/WG04/WG05/WG09/WG16 have been tested for...
After I have done full-length sequencing of your clone, I found it is an alternative splicing form of the gene I am interested in, can you reclone and replace it?
Answer: We will make the best effort to clone the original splicing form and provide you with the replacement clone....
There is one nucleotide change in the sequence, and it encodes an amino acid for proper protein function as sited by many literatures, how will you handle this case?
Answer: Even though we cannot provide replacement clones for this type of cases, we do offer mutagenesis services to...
Since you use PCR method to generate full length ORFs, how will you handle PCR induced mutation?
Answer: Our system incorporated a new high fidelity PCR composition that improves fidelity in generating full length...
How do you explain the differences between your actual sequencing data and GenBank sequences?
Answer: The differences can originate from two potential sources or combination of the two: 1) naturally occurring...
How do you generate full length ORF to produce your clones?
Answer: We use PCR together with several patented technologies to maximize the fidelity of PCR to generate full length...
I would like to sequence the ORFs, what the primer sequences can I use?
Answer: For the ORFEXPRESS™ Shuttle clones, the forwarding sequencing primer (from upstream of 5' end of...
How do you do quality control of your clones?
Answer: During the production of an ORFEXPRESS™ shuttle clone, each full length ORF clone delivered to you...
I cannot find the gene(s) of my interest from searching the database in your website. Can you clone the gene(s) for me? How much will it cost?
Answer: Yes, we will clone the gene(s) for you. It is our mission and continuing effort to clone all full-length ORFs...
In some cases, when comparing your reference sequences with those from NCBI with the same gene symbol or description, I notice there are differing degrees of discrepancies between the sequences. Can you explain the discrepancies?
Answer: There are usually multiple gene transcripts in a gene locus, resulting from alternative splicing, updated...
In the annotation pages for your genes/clones, is the sequence on your website the actual sequence of your clone?
Answer: No, the sequence on the website is not the actual sequence but the reference sequence from NCBI. The actual...
What are the sequences of the Multiple Cloning Site (MCS)?
The sequences are different depending on which full length ORF clone for both ORFEXPRESS™ Gateway® Shuttle...
What is the selection marker for the OmicsLink™ Expression Clones?
Answer: Ampicillin or Neomycin. Please check carefully when you order OmicsLink™ Expression Clones...
What is the selection marker for the ORFEXPRESS™ Gateway® Shuttle Clones?
Answer: Kanamycin.
What is the difference between your OmicsLink™ Expression Clones and expression clones constructed with Gateway® pDEST destination vectors (expression vectors)?
Answer: GCI's OmicsLink™ Expression Clones are generated by its patented RecJoin™ cloning technology....
If I use Gateway® cloning system for 5′ fusion protein, the recombination site (attB) and the Multiple Cloning Site (MCS) in the ORFEXPRESS™ Shuttle Clone will become coding sequence. How many amino acids will be added?
Answer: There are fourteen amino acids between the fusion peptide (or N-terminal Tag) and the ORF encoded protein....
I would like to transfer the ORFs from ORFEXPRESS™ Gateway® Shuttle Clones into my own vectors that have specific features, but these vectors are not Gateway® compatible. How to do it?
Answer: Each ORFEXPRESS™ Shuttle Clone has two multiple cloning sites (MCS) flanking the ORF. By choosing...
How do I remove a stop codon of a full-length ORF that will be used to generate C-terminal tagged fusion protein?
Answer: You can make a point mutation to remove the stop codon. There are more than 35,000 full length ORFs have been...
I would like to use your ORFEXPRESS™ Shuttle clone to express a protein with an N-terminal or C-terminal fusion protein, is that possible?
Answer: Yes, and there are two choices. First, you can use the OmicsLink™ Expression Clones that we have...
How do you select full-length ORFs to be cloned as ORFEXPRESS™ Gateway® Shuttle Clones?
Answer: The selection procedure is very stringent. It includes extraction, comparison and validation of gene sequences...
What type of expression vectors is suitable for the transfer of the ORFs in ORFEXPRESS™ Gateway® Shuttle Clones we are interested in?
Answer: There is a suite of pDEST expression vectors (destination vectors) available from Invitrogen Corporation that...
You claimed that your ORFEXPRESS™ Gateway® Shuttle Clones are Gateway® cloning technology compatible, what does it mean?
Answer: The vector for ORFEXPRESS™ Gateway® Shuttle Clones manufactured by GeneCopoeia, Inc. (GCI) harbors...
Why should I choose full-length ORF clones over other full-length cDNA clones?
Answer: The full-length ORF clones contain only the protein coding sequences, while other full-length whole transcript...
What is a full-length ORF clone?
Answer: A full-length ORF (Open Reading Frame) clone is a plasmid that contains a protein coding DNA insert that...