Since you use PCR method to generate full length ORFs, how will you handle PCR induced mutation?

Answer: Our system incorporated a new high fidelity PCR composition that improves fidelity in generating full length ORFs by 10 fold. It is accomplished by reducing the non-specific binding of primers with template DNA at lower reaction temperature and by reducing the cycles needed for amplifications. We guarantee that there are no frame-shifting changes and no pre-mature stop codon in our full length ORF inserts compared with reference sequences. We will replace the defect clones or refund the charges if that occurs.