All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by GeneCopoeia are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.

I would like to use your ORFEXPRESS™ Shuttle clone to express a protein with an N-terminal or C-terminal fusion protein, is that possible?

Answer: Yes, and there are two choices. First, you can use the OmicsLink™ Expression Clones that we have developed. We have already constructed ORF clones in expression vectors with N-terminal tags or C-terminal tags, such as His tag and eGFP, there are more than 10 types of targs to meet your need. Please check OmicsLink™ product information page (https://www.genecopoeia.com/tech/omicslik/). Second, you may also use Invitrogen's Gateway^® expression vectors for expressing N-terminal or C-terminal fusion proteins. Because our ORFEXPRESS™ Gateway^® Shuttle Clones contains stop codons, you will need to remove the stop codons before transferring ORF inserts into Invitrogen's pDEST expression vectors to produce C-terminal tagged fusion proteins.