How do I remove a stop codon of a full-length ORF that will be used to generate C-terminal tagged fusion protein?

Answer: You can make a point mutation to remove the stop codon. There are more than 35,000 full length ORFs have been constructed with a tag at their 3' ends, such as eGFP and HA, there are more than 10 types you can choose (https://www.genecopoeia.com/tech/omicslink/). You can request us to remove the stop codon and clone it into the vector(s) of your interest at a small fee.