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Protein expression

Answer: You can choose any desired tag for your target protein, as well as decide whether you want the tag to be placed at the N- or C-terminus. You may also use a GeneCopoeia expression construct or provide your own expression clone.

We strongly recommend using a GeneCopoeia OmicsLink™ Expression-Ready ORF clone, which can come with many different tags from which to choose. Our ORFs can be fused with virtually all of the commonly used, powerful tags designed for any application, such as His, eGFP, eYFP, mCherry, Avi, 3xHA, Flag, HaloTag®, etc. In addition, you can choose either fusion expression or non-fusion expression via an Internal ribosome entry site (IRES) or 2A element, which permit protein co-expression, so that you can monitor precise gene expression. With an IRES or 2A element, you can monitor the accurate expression of a native protein without any potentially negative effects caused by fusion tags.

Answer: We offer pre-made recombinant protein products. Our expression facility uses host cell systems such as bacteria, mammalian cell lines (typically HEK293T cells), yeast, insect and cell-free expression systems. With fully sequence-verified expression clones from GeneCopoeia, we help researchers save time for further research.
Answer: GeneCopoeia provides pre-made recombinant proteins in standard amounts of either 25 µg or 100µg. You may also request custom amounts. Please contact technical support to get more information.
Answer: The concentration of GeneCopoeia recombinant protein is determined using the BCA assay.
Answer: Yes. GeneCopoeia recombinant proteins are produced serum-free. In addition, most of GeneCopoeia’s proteins are carrier-free (except for proteinase products). They do not contain BSA, and therefore can be used directly in SDS PAGE gels and western blots.
Answer: Yes. Removing a tag can be as easy as choosing one. The removal of some small molecular weight tags, such as His and Flag, is often not necessary.
Answer: GeneCopoeia uses either affinity purification or ion exchange chromatography for protein purification. We will determine the best purification strategy based on your needs and the protein’s properties.
Answer: GeneCopoeia recombinant proteins have not been functionally tested in vivo. Therefore, GeneCopoeia cannot guarantee that all proteins are fully functional in all assays, including those for in vivo applications.
Answer: GeneCopoeia recombinant proteins generally remain stable for 1-3 years under appropriate storage conditions. We recommend aliquotting the protein solution and storing the tubes at -80℃. Avoid repeated freeze-thaw cycles.