Answer: There are usually multiple gene transcripts in a gene locus, resulting from alternative splicing, updated information about ORFs, polymorphism, or redundancies from duplication of cloning effort from different laboratory groups. During the process of selecting full-length genes for ORF cloning, we try to choose the most representative transcript from each gene locus, which may differ slightly or significantly from other transcripts in the same gene locus. This approach provides the broadest coverage of all gene loci. In many instances, we do obtain multiple variants or versions of full length ORFs from our cloning production pipeline. Customers are encouraged to send inquires to inquiry@genecopoeia.com if he/she is interested in obtaining a particular variant/version of a gene/ORF.
In some cases, discrepancies could be originated from changed annotation of ORF start and/or stop location as the result of newly available sequence information and/or experimental evidence. On regular basis, we check for information updates on ORF annotations in public databases such as NCBI and EMBL. The new ORF annotations will then be used in our recloning effort to keep our ORF clones most updated.