Answer: The vector for ORFEXPRESS™ Gateway® Shuttle Clones manufactured by GeneCopoeia, Inc. (GCI) harbors full length ORFs flanked by attL1 and attL2 recombination sites that pair with the Gateway® pDEST expression vectors containing attR1 and attR2 recombination sites. When GCI's ORFEXPRESS™ Shuttle Clones are mixed with Gateway® compatible pDEST expression vector(s), with optimal concentrations of enzymes and conditions, the ORFs in the ORFEXPRESS™ Shuttle clones can be transferred into Gateway® pDEST expression vector(s). Because of the unique feature of recombination sites in the vectors, the transferred ORFs will be under the control of the promoter(s) in the destination expression vector(s).