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Manuals, Protocols, and MSDS

by | Mar 8, 2012 | Resources

Clone datasheets are typically emailed upon shipment. If you did not receive the datasheet(s) for your order, please check your spam or junk email and/or with the person who placed the order. You may also complete the following online form here or email orders@genecopoeia.com for data requests. A Datasheet Access system for budget-friendly ORF and miRNA clones is also available. For all other kit and reagent manuals and clone protocols, please find them below.

 
Clones ORF cDNA
Brand Name Product Name Catalog Number Manual/Datasheet MSDS

OmicsLink™

ORFEXPRESS™

ORFeome

 GeneCopoeia Full-Length cDNA Clones FL
ORFEXPRESS™ Gateway® PLUS Shuttle Clones GC-
ORFeome Collaboration Clones in Gateway® Entry Vector HOC / MOC
OmicsLink™ Expression Ready ORF cDNA Clones EX-
OmicsLink™ Expression Ready ORF Lentiviral cDNA Clones EX-_____-Lv
  miRNA 3’UTR target
Brand Name Product Name Catalog Number Manual/Datasheet MSDS
miTarget™ miTarget™ MicroRNA 3′ UTR Target Clones HmiT / MmiT / RmiT N/A
  miRNA inhibitor
Brand Name Product Name Catalog Number Manual/Datasheet MSDS
miArrest™ miArrest™ MicroRNA Inhibitors HmiR-AN / MmiR-AN / RmiR-AN
  miRNA mimic
Brand Name Product Name Catalog Number Manual/Datasheet MSDS
miExpress™ miExpress™ MicroRNA mimics HmiR-SN / MmiR-SN / RmiR-SN
  Promoter Reporter
Brand Name Product Name Catalog Number Manual/Datasheet MSDS
GLuc-ON™  GLuc-ON™ Promoter Reporter Clones HPRM / MPRM N/A
  shRNA
Brand Name Product Name Catalog Number Manual/Datasheet MSDS
OmicsLink™ OmicsLink™ shRNA Clone Collections HSH / MSH / RSH N/A
  siRNA oligos
Product Name Catalog Number Manual/Datasheet MSDS
siRNA oligos Hsi / Msi / Rsi
  CRISPR
Brand Name Product Name Catalog Number Manual/Datasheet MSDS
GeneHero™ GeneHero™ Cas9 and sgRNA Clone Collections HCP / MCP / RCP
  TALEN
Brand Name Product Name Catalog Number Manual/Datasheet MSDS
Genome-TALER™ Genome-TALER™ TALEN Clone Collections HTN / HTF / MTN / MTF / RTN / RTF N/A

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by | Mar 8, 2012 | Resources

 

All-in-One™ miRNA qRT-PCR Reagent Kits and Validated Primers

by | Mar 8, 2012 | Products

All-in-One™ miRNA qRT-PCR Detection Kit 2.0
All-in-One™ miRNA qRT-PCR Detection Kit 2.0
All-in-One™ miRNA qRT-PCR Detection Kit 2.0
All-in-One™ miRNA qRT-PCR Detection Kit 2.0
All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0 for miRNA qPCR array
All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0 for miRNA qPCR array
All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0 for miRNA qPCR array
All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0 for miRNA qPCR array
All-in-One™ miRNA Universal Adaptor PCR Primer
All-in-One™ miRNA Universal Adaptor PCR Primer

All-in-One™ miRNA qRT-PCR Detection Kit 2.0 All-in-One™ miRNA qRT-PCR Detection Kit 2.0 All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0 for miRNA qPCR array All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0 for miRNA qPCR array All-in-One™ miRNA Universal Adaptor PCR Primer

Price: $427.00 $1,065.00 $243.00 $557.00 $63.00
Catalog#:
  • QP115
  • QP116
  • QP117
  • QP118
  • QP029
Size: 20 RT and 200 qPCR reactions 60 RT and 600 qPCR reactions 20 RT reactions 60 RT reactions 50 µM ; 20 µl
Qty:
Manual: DownloadDownloadDownloadDownloadDownload
View your cart


Introduction
The All-in-One™ miRNA qRT-PCR reagent kits include the All-in-One™ miRNA qRT-PCR Detection Kits 2.0 and the All-in-One™ miRNA First-Strand cDNA Synthesis Kits 2.0. When used with All-in-One™ Validated miRNA Primers or miProfile™ miRNA qPCR Arrays, the result is high-performance expression-profiling of all miRNAs.
A novel optimized blend of poly A polymerase and reverse transcriptase with an PAP/RT buffer that was specifically developed for the All-in-One Reverse Transcriptase Mix delivers maximum activity of both enzymes for robust first-strand cDNA synthesis from mature miRNAs. The All-in-One™ First-Strand cDNA Synthesis Kit 2.0 is optimized for use with miProfile qPCR Arrays.
The All-in-One™ RT-PCR Detection Kit 2.0 includes both RT and PCR reagents. It combines PCR technology and SYBR® Green to make fast and accurate quantification of mature miRNAs from as little as 10 pg of small RNA or 20 pg of total RNA samples. The kit is designed for use with All-in-One Validated miRNA Primers.

All-in-One™ miRNA assays provides sensitivity and specificity you can afford

  • Detect miRNAs from as little as 10 pg of small RNA or 20 pg of total RNA
  • Compatible with GeneCopoeia miRNA qPCR arrays or validated miRNA-specific primers

All-in-One™ miRNA assays are fast and easy

Figure 1. Overview of steps involved in the All-in-One™ miRNA qRT-PCR Detection Kit 2.0. During 3′ polyadenylation, M-MLV RTase and a unique Oligo dT Adaptor primer, reverse transcribes the poly A miRNAs. The kit qRT-PCR Mix, containing SYBR® Green specifically detects the reverse transcribed miRNAs.
 

Combine these tools with GeneCopoeia miRNA plasmid constructs and assays for comprehensive functional analysis studies.

 

*miRNA-specific primers sold separately.

Budget-friendly miRNA clones  $200 & up
For a comprehensive overview of GeneCopoeia microRNA functional analysis tools- Click here!
Trademarks: SYBR® (Molecular Probes)

RT-PCR kits

   
 + Download miRNA qPCR primer validation report sample (PDF)
 

QuantiTaq™ Thermostable DNA Polymerase

by | Mar 8, 2012 | Products

QuantiTaq™ DNA Polymerase is an ultra-pure, thermostable DNA Polymerase purified through many steps of optimized chromatography. Our production and quality control processes ensure utmost purity, reproducibility, and processivity. The enzyme meets the requirements of specialized PCR with a linear amplification manner and highly reproducible when a template is unlimited diluted. QuantiTaq™ DNA Polymerase is packaged with 10X Reaction Buffer and Dilution Buffer. Application: DNA amplification via PCR; Primer extension for probe labeling; RT-PCR; AmpArray™ gene expression profiling.
Features: High purity; Ultra reproducible results; Robotic use.
Storage Buffer: 20 mM Tris-HCl pH 8.0 , 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, and 0.5% Tween 20 and other integrated components for stability of long time storage.
Dilution buffer: 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, and other integrated components for precise and easy aliquot to assemble reactions.
Reaction Buffer:10x Reaction Buffer with Mg2+: 500 mM KCl, 100 mM Tris-HCl pH 8.5 (at 25°C), 15mM Mg++ and other integrated components for robotic use.
Unit Definition: One unit of either enzyme will incorporate 10 nmol of total dNTPs in 30 minutes at 72°C.
Associated Activities: Endonuclease, exonuclease, and DNA ligation activities were not detectable after one or two hours of incubation time, respectively, of 1 µg Lamda DNA and 0.22 µg of EcoRI-digested Lamda DNA at 72°C in the presence of 15-20 units of QuantiTaq™ DNA Polymerase.
The total RNA (10µg) was transcribed by reverse transcriptase in 100 µl reaction mixture. After diluted 5, 25, 125, 625, 3125 and 15625 fold (as shown in wells of columns 1, 2, 3, 4, 5, and 6 above, respectively), 2µl of diluted reaction mixture was added to the 23µl of PCR reaction mixture containing indicated Taq DNA polymerases. PCR was performed: 94°C for 3′ followed by 30 cycles (94°C for 30″, 62°C for 30″ 72°C for 45″).
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Fig. 1. Amplification of human G3PDH with first strand cDNA from T-cell by QuantiTaq™ and various Taq DNA polymerases from other suppliers.

miArrest™ microRNA synthetic inhibitors

by | Mar 8, 2012 | Products

*Promotions are valid in the US & Canada only. For international customers, please contact your local distributors. Discounts are not valid on previous purchases and cannot be combined with additional discounts.
Ideal for transient transfection, synthetic miRNA inhibitors are chemically synthesized and sequence-specific single-stranded nucleic acid molecules that are designed and optimized to bind to specific miRNA molecules. Upon binding, they block the effect of miRNA molecules on their targeted genes with high specificity and efficiency.

​

Features

  • Pre-designed inhibitors for all known human, mouse and rat miRNAs
  • Chemically enhanced to improve efficiency and longevity
  • Ready to transfect oligonucleotides that inhibit specific endogenous miRNA
  • Available as individual or full sets for miRNA screening
​ Effect of synthetic oligonucleotide inhibitor against miR-125a Figure 1. Data showing the effect of miArrest miRNA synthetic inhibitor in a dose-dependent manner. Varying amounts of synthetic miR-125a inhibitor (GeneCopoeia HmiR-AN0094-SN were co- transfected into HEK 293 cells with 1) a miR-125a precursor expression plasmid (GeneCopoeia HmiR0309-MR03) and 2) a miRNA target sequence expression clone expression LIN28, a known target gene for miR-125a (GeneCopoeia HmiT019205-MT01: 3′-UTR sequence of LIN28 in FLuc-RLuc dual reporter expression vector). Both Firefly luciferase activity and an internal Renilla luciferease activity were determined 24 hours post-transfection. The activity ratio of FLuc to RLuc was set to 1 for the single transfection sample with LIN28 3′-UTR target sequence expression clone (left-most bar), against which the activities of other samples were normalized. The result shows that mir-125a suppressed the luciferase activity from the Fluc-LIN28-3′-UTR clone by more than 60% (Bar 2 from left). This suppression effect was blocked by the introduction of varying amount of miArrest™ inhibitor clone against miR-125a in a dose-dependent manner.

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Publications using GeneCopoeia microRNA solutions