Monthly Archives: March 2012

shRNA Clone Collection Publications

View selected publications citing GeneCopoeia's OMICSLINK™ shRNA clone sets from the recent literature 2021 Mao, Y., et al. (2021). Aberrantly up-regulated miR-142-3p inhibited the proliferation and invasion of trophoblast cells by regulating FOXM1. Placenta doi: 10.1016/j.placenta.2021.01.002 [OMICSLINK™ FOXM1 shRNA clone set] You, L-N., et al. (2021). Exosomal LINC00161 promotes angiogenesis and metastasis via regulating miR-590-3p/ROCK axis in hepatocellular carcinoma. Cancer Gene Therapy doi: 10.1038/s41417-020-00269-2 [OMICSLINK™ LINC00161 shRNA clone se......
Posted in Publications by Technology Area

Reagents and Kits Publications

2016 Bid, HK. et al. (2016) The Bromodomain BET inhibitor JQ1 Suppresses Tumor Angiogenesis in Models of Childhood Sarcoma. Molecular Cancer Therapeutics,Published OnlineFirst February 23, 2016; doi: 10.1158/1535-7163.MCT-15-0567. [AP-1 activity assay kit] Wang, S., et al. (2016). Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. Acta Pharmacologica Sinica doi: 10.1038/aps.2016.118 [RNAzol®RT reagent]   2015 Rosen......
Posted in Publications by Technology Area

qPCR Primers and Kits Publications

View selected publications citing GeneCopoeia's qPCR-related products from the recent literature 2021 Wu, D., et al. (2020). Long non-coding RNA GClnc1 knockdown suppresses progression of epithelial ovarian cancer by recruiting FOXC2 to disrupt the NOTCH1/NF-κB/Snail pathway. Experimental Cell Research doi: 10.1016/j.yexcr.2020.112422 [BlazeTaq™ One-Step SYBR Green RT-qPCR Kit] Duan, J., et a. (2021). miR-152/TNS1 axis inhibits non-small cell lung cancer progression through Akt/mTOR/RhoA pathway. Bioscience Reports doi: 10.1042/BSR20201539 [All-in-One™ miRN......
Posted in Technologies

ORF cDNA Clone Publications

View selected publications citing GeneCopoeia's ORF cDNA clones from the recent literature 2021 Xiang, H., et al. (2021). miR-124-3p combined with miR-506-3p delay hepatic carcinogenesis via modulating sirtuin. Biomarkers doi: 10.1080/1354750X.2020.1854856 [OMICSLINK™ SIRT1 ORF expression clone] Yoon, S., et al. (2021). Homer1 promotes dendritic spine growth through ankyrin-G and its loss reshapes the synaptic proteome. Molecular Psychiatry doi: 10.1038/s41380-020-00991-1 [OMICSLINK™ abkyrin-G ORF expression clone] Lv, X., et al. (2021). Negative Feedback Loop Be......
Posted in Publications by Technology Area

miRNA Complete Solutions Publications

View selected publications citing GeneCopoeia's miRNA-related products from the recent literature 2021 Duan, J., et a. (2021). miR-152/TNS1 axis inhibits non-small cell lung cancer progression through Akt/mTOR/RhoA pathway. Bioscience Reports doi: 10.1042/BSR20201539 [All-in-One™ miRNA miRNA extraction kit; All-in-One™ miRNA qPCR Detection kit] Tang, C., et al. (2021). LncRNA MAFG-AS1 regulates miR-125b-5p/SphK1 axis to promote the proliferation, migration, and invasion of bladder cancer cells. Human Cell doi: 10.1007/s13577-020-00470-3 [All-in-One™ miRNA q......
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Lentiviral Solutions Publications

View selected publications citing GeneCopoeia's lentiviral products from the recent literature 2021 Joshi, S., et al. (2021). ACE2 Gene Transfer Ameliorates Vasoreparative Dysfunction in CD34+ Cells Derived from Diabetic Older Adults. Clinical Science doi: 10.1042/CS20201133 [Lentifect™ Lentiviral particles expressing ACE2 promoter] Hyroššová, P., et al. (2021). PEPCK-M recoups tumor cell anabolic potential in a PKC-ζ-dependent manner. Cancer and Metabolism doi: 10.1186/s40170-020-00236-3 [Lentifect™ Lentiviral particles expressing PCK1 ......
Posted in Publications by Technology Area

Recombination Cloning Technology

Recombination cloning technology is based on the site specific recombination reaction of phage lambda, and is the same principle as Gateway® technology, an efficient biochemical process that conserves genetic information (see Figure 1). The transmission of an ORF from EZShuttle Gateway® PLUS ORF Clones to a pEZ™ or Gateway® Destination Vector is a one step simple and rapid operation (60 minutes at room temperature), which saves a significant amount of time and effort from the tedious, multiple step, sub-cloning process involving enzyme digestions, ligations, gel electro......
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A Simple Way to Construct Desirable Expression Clones

The reaction where the ORFEXPRESS™-Shuttle Clone recombines with the Destination Vector to generate the Expression Clone is based on the attL x attR excision reaction. This recombination reaction is conservative in the sense that there is no net addition or loss of base pairs during the LXR reaction and it is highly specific. The coding region of a gene in an ORFEXPRESS™-Shuttle Clone is flanked by attL1 and attL2 at upstream of ATG (the start codon) and downstream of TGA (the stop codon), respectively. There are attR1 and attR2 recombonation s......
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The Principle of Gateway Cloning Technology

This cloning technology is based on the nature of site-specific recombination, the integrative and excisive recombination reactions between chromosomes of Bacteriophage lambda and E. coli bacterium. The integrative recombination is catalyzed by Int (integrase) and IHF (Integration Host Factor). The recombination between attB and attP sites results in attL and attR sites that flank the integrated lambda DNA. Three proteins (IHF, Int and Xis) are required for the excisive recombination. The attL and attR sites located at both sides of the inserted phage geno......
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Applications of OmicsLink™ Clones and Comparison with Whole Transcript cDNA Clones

Express proteins in E.coli cells Express proteins in mammalian cells by reverse transfection Produce proteins in in vitro cell-free translation systems Express proteins with unique tag that facilitates purification and down-stream analysis Generate probes of in situ hybridization for tissue or organ gene expression profiling Produce templates for esiRNA for gene silencing functional analysis Validation of shRNA/miRNA function     Clone Characteristics ......
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