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A Simple Way to Construct Desirable Expression Clones

by | Mar 8, 2012 | Technologies

The reaction where the ORFEXPRESS™-Shuttle Clone recombines with the Destination Vector to generate the Expression Clone is based on the attL x attR excision reaction. This recombination reaction is conservative in the sense that there is no net addition or loss of base pairs during the LXR reaction and it is highly specific.

The coding region of a gene in an ORFEXPRESS™-Shuttle Clone is flanked by attL1 and attL2 at upstream of ATG (the start codon) and downstream of TGA (the stop codon), respectively. There are attR1 and attR2 recombonation sites in the Destination Vector of Invitrogen Corporation's Gateway® Technology. The recombination reactions of attL1 x attR1 and attL2 x attR2 which occur between the ORFEXPRESS™-Shuttle Clone and Destination Vector are catalyzed by an enzyme mixture composed of three proteins, IHF, Int and Xis, forming an Expression Clone which contains the ORF in the desired host vector.

To select suitable Expression Vector(s) for different hosts, visit Gateway® Technology at Invitrogen Corporation's home page, www.invitrogen.com.

 

The Principle of Gateway Cloning Technology

by | Mar 8, 2012 | Technologies

 

 

This cloning technology is based on the nature of site-specific recombination, the integrative and excisive recombination reactions between chromosomes of Bacteriophage lambda and E. coli bacterium. The integrative recombination is catalyzed by Int (integrase) and IHF (Integration Host Factor). The recombination between attB and attP sites results in attL and attR sites that flank the integrated lambda DNA.

 

Three proteins (IHF, Int and Xis) are required for the excisive recombination. The attL and attR sites located at both sides of the inserted phage genome DNA recombine site-specifically during the excision event, reforming the attP site in lambda and the attB site in the E. coli chromosome. These biochemical reaction processes, performed in vitro, are the basis of the Gateway® Cloning Technology.
 

Applications of OmicsLink™ Clones and Comparison with Whole Transcript cDNA Clones

by | Mar 8, 2012 | Technologies

  • Express proteins in E.coli cells
  • Express proteins in mammalian cells by reverse transfection
  • Produce proteins in in vitro cell-free translation systems
  • Express proteins with unique tag that facilitates purification and down-stream analysis
  • Generate probes of in situ hybridization for tissue or organ gene expression profiling
  • Produce templates for esiRNA for gene silencing functional analysis
  • Validation of shRNA/miRNA function
  

 

Clone Characteristics
OmicsLink™ ORF clones
Whole Transcript cDNA Clones
Gene transcript ORF (Start to stop only) Whole transcripts including 5' and 3' UTRs
Expression ready Yes Some are, most will need further subcloning procedures to remove 5' and 3' UTRs
Mammalian cell transfection
– Transicent Transfection
– Stable Transfection		 Yes
Yes		 Only a small number No
Unique protein tags
– Protein-Molecule interactions
– Concentration of lowly expressed proteins
– Affinity purification of expressed proteins		 Yes
Yes
Yes
Yes		 No
No
No
No
shRNAi validation and rescue Yes No
miRNA and miRNA target validation and rescue Yes No
Produce proteins via in vitro cell-free translation systems Yes No
production Template for esiRNA Yes No
Probe generations for in situ hybridization Yes No

 

OmicsLink™ Expression-Ready ORF cDNA Vectors

by | Mar 8, 2012 | Resources

  • Expression-ready so no further sub cloning necessary
  • Sequence-verified and available for 40,000+ human and mouse genes
  • Choices of 150+ expression vectors as listed in the tables below
  • Choices of untagged clones for native protein expression
  • Choices of 50+ fusion tags with or without IRES as listed below
  • Gateway PLUS™ shuttle system also available as shown below
 

   
Need assistance selecting a vector system? Contact us here or email directly at inquiry@genecopoeia.com

Expression vectors and Gateway PLUS™ shuttle system for your gene(s) of interest

Vector Promoter Host Cell Selection Marker Tag Protease Site
pShuttle™ N/A Gateway PLUS shuttle N/A Untagged N/A
pShuttle ™ N/A Gateway PLUS shuttle
(no stop codon)		 N/A Untagged N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-B01 T7 Bacterial N/A N-His N/A
pReceiver-B31 T7 Bacterial N/A C-His N/A
pReceiver-B02 T7 Bacterial N/A Untagged N/A
pReceiver-B03 T7 Bacterial N/A N-GST Tev
pReceiver-B04 T7 Bacterial N/A N-GST EK
pReceiver-B05 Tac Bacterial N/A N-GST Tev
pReceiver-B06 Tac Bacterial N/A N-GST EK
pReceiver-B07 Tac Bacterial N/A N-MBP Tev
pReceiver-B08 Tac Bacterial N/A N-MBP EK
pReceiver-B09 T7 Bacterial N/A N-Avi N/A
pReceiver-B10 Tac Bacterial N/A N-Flag N/A
pReceiver-B11 Tac Bacterial N/A N-His N/A
pReceiver-B12 Tac Bacterial N/A His-SUMO Sumo protease
pReceiver-B13 T7 Bacterial N/A His-SUMO SUMO Protease
pReceiver-B80 T7 Bacterial N/A N-HaloTag Tev
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-M34 CAG Mammalian Neomycin C-Flag N/A
pReceiver-M35 CMV Mammalian Neomycin C-Flag N/A
pReceiver-M37 CAG Mammalian Neomycin C-eGFP N/A
pReceiver-M39 CMV Mammalian Puromycin C-Flag N/A
pReceiver-M62 CMV Mammalian Neomycin C-Avi-IRES-Biotin ligase N/A
pReceiver-M73 CMV Mammalian Neomycin C-Myc-IRES2-mCherry N/A
pReceiver-M83 CMV Mammalian Neomycin IRES2-mCherry N/A
pReceiver-M90 CMV Mammalian Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-M91 CMV Mammalian Puromycin C-3xHA-SV40-eGFP-IRES-Puromycin N/A
pReceiver-M92 CMV Mammalian Puromycin C-Myc-SV40-eGFP-IRES-Puromycin N/A
pReceiver-M93 CMV Mammalian Puromycin C-Flag-SV40-eGFP-IRES-Puromycin N/A
pReceiver-M94 EF1a Mammalian Puromycin Untagged N/A
pReceiver-M95 CMV Mammalian Puromycin SV40-mCherry-IRES-Puromycin N/A
pReceiver-M97 EF1a Mammalian Neomycin C-eGFP(monomeric) N/A
pReceiver-M99 CMV Mammalian Neomycin C-mEOS3 N/A
pReceiver-M01 CMV Mammalian Neomycin N-His N/A
pReceiver-M51 CMV Mammalian Neomycin C-His+IRES-eGFP N/A
pReceiver-M77 CMV Mammalian Neomycin C-His N/A
pReceiver-M02 CMV Mammalian Neomycin Untagged N/A
pReceiver-M67 CMV Mammalian Hygromycin Untagged N/A
pReceiver-M68 CMV Mammalian Puromycin Untagged N/A
pReceiver-M29 CMV Mammalian Neomycin N-eGFP N/A
pReceiver-M03 CMV Mammalian Neomycin C-eGFP N/A
pReceiver-M98 CMV Mammalian Neomycin C-eGFP(monomeric) N/A
pReceiver-M15 CMV Mammalian Neomycin N-eYFP N/A
pReceiver-M16 CMV Mammalian Neomycin C-eYFP N/A
pReceiver-M32 CMV Mammalian Neomycin N-eCFP N/A
pReceiver-M33 CMV Mammalian Neomycin C-eCFP N/A
pReceiver-M04 CMV Mammalian Neomycin N-GST EK
pReceiver-M05 CMV Mammalian Neomycin N-Avi N/A
pReceiver-M48 CMV Mammalian Neomycin N-Avi+IRES-Biotin ligase N/A
pReceiver-M17 CMV Mammalian Neomycin C-Avi N/A
pReceiver-M06 CMV Mammalian Neomycin N-3xHA N/A
pReceiver-M07 CMV Mammalian Neomycin C-3xHA N/A
pReceiver-M08 CMV Mammalian Neomycin C-3xHA-His N/A
pReceiver-M43 CMV Mammalian Neomycin N-Myc N/A
pReceiver-M45 CMV Mammalian Neomycin C-3xHA+IRES-eGFP N/A
pReceiver-M09 CMV Mammalian Neomycin C-Myc N/A
pReceiver-M10 CMV Mammalian Neomycin C-Myc-His N/A
pReceiver-M72 CMV Mammalian Neomycin C-Myc+IRES2-eGFP N/A
pReceiver-M11 CMV Mammalian Neomycin N-Flag N/A
pReceiver-M12 CMV Mammalian Neomycin N-3XFlag N/A
pReceiver-M46 CMV Mammalian Neomycin C-Flag+IRES-eGFP N/A
pReceiver-M14 CMV Mammalian Neomycin C-3XFlag N/A
pReceiver-M49 CMV Mammalian Neomycin N-HaloTag Tev protease
pReceiver-M50 CMV Mammalian Neomycin C-HaloTag Tev protease
pReceiver-M55 CMV Mammalian Neomycin N-mCherry N/A
pReceiver-M56 CMV Mammalian Neomycin C-mCherry N/A
pReceiver-M61 CMV Mammalian Neomycin IRES2-eGFP N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-Lv117 CMV Lentiviral Puromycin N-3xHA N/A
pReceiver-Lv118 CMV Lentiviral Neomycin N-3xHA N/A
pReceiver-Lv145 EF1a Lentiviral Hygromycin SV40-hygromycin N/A
pReceiver-Lv163 EF1a Lentiviral Neomycin SV40-neomycin N/A
pReceiver-Lv170 CMV Lentiviral Neomycin C-Myc N/A
pReceiver-Lv181 CMV Lentiviral Puromycin N-3xFlag N/A
pReceiver-Lv185 PGK Lentiviral Puromycin Untagged N/A
pReceiver-Lv188 CMV Lentiviral N/A C-eGFP N/A
pReceiver-Lv193 CMV Lentiviral Puromycin C-eGFP(monomeric) N/A
pReceiver-Lv195 EF1a Lentiviral Puromycin SV40-puromycin N/A
pReceiver-Lv196 TRE3G Lentiviral Puromycin SV40-puromycin Tet-On 3G System
pReceiver-Lv197 CMV Lentiviral Blasticidin Untagged N/A
pReceiver-Lv208 TRE3G Lentiviral Puromycin SV40-rtTA-T2A-eGFP-IRES-Puro Tet-On 3G System
pReceiver-Lv209 TRE3G Lentiviral Puromycin SV40-eGFP-IRES-Puro Tet-On 3G System
pReceiver-Lv212 TRE3G Lentiviral Puromycin SV40-rtTA-IRES-Puro Tet-On 3G System
pReceiver-Lv218 CMV Lentiviral Neomycin IRES2-Neomycin N/A
pReceiver-Lv219 CMV Lentiviral Puromycin IRES2-Puromycin N/A
pReceiver-Lv221 CMV Lentiviral N/A IRES2-eYFP N/A
pReceiver-Lv222 CMV Lentiviral N/A IRES2-eCFP N/A
pReceiver-Lv233 EF1a Lentiviral Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv234 CAG Lentiviral N/A IRES2-eGFP N/A
pReceiver-Lv235 CAG Lentiviral Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv236 CAG Lentiviral Puromycin Untagged N/A
pReceiver-Lv237 CAG Lentiviral Puromycin C-Flag-SV40-Puromycin N/A
pReceiver-Lv241 CMV Lentiviral Puromycin C-Flag-SV40-Puromycin N/A
pReceiver-Lv243 CAG Lentiviral Puromycin C-Avi-Flag-SV40-Puromycin N/A
pReceiver-Lv244 CMV Lentiviral Hygromycin SV40-mCherry-IRES-Hygromycin N/A
pReceiver-Lv245 EF1a Lentiviral Puromycin cMyc-SV40-mCherry-IRES-Puromycin N/A
pReceiver-Lv246 UBC Lentiviral Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv247 UBC Lentiviral Puromycin IRES2-mCherry-IRES-Puromycin N/A
pReceiver-Lv248 TRE3G Lentiviral Puromycin SV40-tTA-T2A-eGFP-IRES-Puro Tet-Off System
pReceiver-Lv251 CMV Lentiviral Puromycin C-3XFlag-SV40-Puromycin N/A
pReceiver-Lv253 EF1a Lentiviral Puromycin SV40-mcherry-IRES-Puromycin N/A
pReceiver-Lv254 EF1a Lentiviral Neomycin SV40-mcherry-IRES-Neomycin N/A
pReceiver-Lv105 CMV Lentiviral Puromycin Untagged N/A
pReceiver-Lv151 CMV Lentiviral Neomycin Untagged N/A
pReceiver-Lv152 CMV Lentiviral Hygromycin Untagged N/A
pReceiver-Lv153 CMV Lentiviral N/A Untagged N/A
pReceiver-Lv186 CMV Lentiviral N/A C-3xHA N/A
pReceiver-Lv157 CMV Lentiviral Neomycin C-3xHA N/A
pReceiver-Lv120 CMV Lentiviral Puromycin C-3xHA N/A
pReceiver-Lv108 CMV Lentiviral Puromycin N-Avi N/A
pReceiver-Lv124 CMV Lentiviral Puromycin C-Avi N/A
pReceiver-Lv242 CMV Lentiviral Puromycin C-Avi-FLAG N/A
pReceiver-Lv154 CMV Lentiviral Neomycin C-eCFP N/A
pReceiver-Lv127 CMV Lentiviral Puromycin C-eCFP N/A
pReceiver-Lv109 CMV Lentiviral Puromycin N-eCFP N/A
pReceiver-Lv103 CMV Lentiviral Puromycin N-eGFP N/A
pReceiver-Lv122 CMV Lentiviral Puromycin C-eGFP N/A
pReceiver-Lv182 CMV Lentiviral Neomycin N-eGFP N/A
pReceiver-Lv183 CMV Lentiviral Neomycin C-eGFP N/A
pReceiver-Lv189 CMV Lentiviral N/A N-eGFP N/A
pReceiver-Lv123 CMV Lentiviral Puromycin C-eYFP N/A
pReceiver-Lv104 CMV Lentiviral Puromycin N-eYFP N/A
pReceiver-Lv187 CMV Lentiviral N/A C-FLAG N/A
pReceiver-Lv101 CMV Lentiviral Neomycin N-Flag N/A
pReceiver-Lv102 CMV Lentiviral Puromycin N-Flag N/A
pReceiver-Lv158 CMV Lentiviral Neomycin C-FLAG N/A
pReceiver-Lv203 CMV Lentiviral Puromycin C-Flag-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv216 CMV Lentiviral Puromycin C-Flag-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv129 CMV Lentiviral Puromycin C-HaloTag Tev
pReceiver-Lv110 CMV Lentiviral Puromycin N-HaloTag Tev
pReceiver-Lv211 CMV Lentiviral Hygromycin C-HaloTag-IRES-hygromycin N/A
pReceiver-Lv128 CMV Lentiviral Puromycin C-His N/A
pReceiver-Lv215 CMV Lentiviral N/A IRES2-eGFP N/A
pReceiver-Lv205 CMV Lentiviral Puromycin IRES2-eGFP-IRES-puromycin N/A
pReceiver-Lv220 CMV Lentiviral Hygromycin IRES2-Hygromycin N/A
pReceiver-Lv214 CMV Lentiviral N/A IRES2-mCherry N/A
pReceiver-Lv213 CMV Lentiviral Puromycin IRES2-mCherry-IRES-puromycin N/A
pReceiver-Lv111 CMV Lentiviral Puromycin N-mCherry N/A
pReceiver-Lv130 CMV Lentiviral Puromycin C-mCherry N/A
pReceiver-Lv155 CMV Lentiviral Neomycin C-mCherry N/A
pReceiver-Lv107 CMV Lentiviral Puromycin N-Myc N/A
pReceiver-Lv125 CMV Lentiviral Puromycin C-Myc N/A
pReceiver-Lv126 CMV Lentiviral Puromycin C-Myc-His N/A
pReceiver-Lv202 CMV Lentiviral Puromycin C-Myc-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv204 CMV Lentiviral Puromycin C-Myc-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv207 CMV Lentiviral Hygromycin SV40-eGFP-IRES-hygromycin N/A
pReceiver-Lv201 CMV Lentiviral Puromycin SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv217 CMV Lentiviral Puromycin SV40-hLUC-IRES-puromycin N/A
pReceiver-Lv206 CMV Lentiviral Puromycin SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv171 EF1a Lentiviral N/A IRES-eGFP N/A
pReceiver-Lv173 EF1a Lentiviral N/A IRES-mcherry N/A
pReceiver-Lv180 EF1a Lentiviral Puromycin IRES-mcherry-SV40-puromycin N/A
pReceiver-Lv179 EF1a Lentiviral Puromycin IRES-eGFP-SV40-puromycin N/A
pReceiver-Lv228 PGK Lentiviral N/A IRES2-eGFP N/A
pReceiver-Lv229 PGK Lentiviral N/A IRES2-mcherry N/A
pReceiver-Lv230 PGK Lentiviral Puromycin IRES2-mcherry-IRES-puromycin N/A
pReceiver-Lv231 PGK Lentiviral Puromycin IRES2-eGFP-IRES-puromycin N/A
pLX304 CMV Lentiviral (ORFeome) Blasticidin C-V5 N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site

search clone

If none of our ORF expression-ready AAV vectors is suitable for your research, please use GeneCopoeia’s V-Architect tools which provides various promoters and tags to match your experimental design.
Vector name Promoter Promoter description Reporter gene Max length of insertion
pEZ-AV02 EF-1α Broad scope of application. Better choice than CMV for in vivo applications. N/A 3.2 kb
pEZ-AV04 CAG Ubiquitous. Better choice than CMV for in vivo applications. N/A 2 kb
pEZ-AV06 CBh Hybrid chicken beta actin promoter. Provides better long-term expression than CMV in neural cells. N/A 2.8 kb
pEZ-AV07 CMV Strong promoter, broad scope of application. IRES-eGFP 1.3 kb
pEZ-AV08 EF-1α Broad scope of application. Better choice than CMV for in vivo applications. IRES-eGFP 2 kb
pEZ-AV09 CAG Ubiquitous. Better choice than CMV for in vivo applications. IRES-eGFP 0.9 kb
pEZ-AV11 CBh Hybrid chicken beta actin promoter. Provides better long-term expression than CMV in neural cells. IRES-eGFP 1.7 kb
pEZ-AV12 CAMKlla Highly-specific expression in excitatory neurons in the neocortex and hippocampus. N/A 2.4 kb
pEZ-AV13 hSYN Human synapsin-1 gene promoter, neuronal-specific. N/A 3.2 kb
pEZ-AV15 MHC Muscle-specific human promoter. N/A 3.3 kb
pEZ-AV18 MCK Cardiac muscle-specific human promoter. N/A 3.0 kb
pEZ-AV24 MBP Oligodendrocyte-specific expression in central nervous system. N/A 3.0 kb
pEZ-AV33 ALB Liver-specific human promoter. N/A 2.0 kb
pEZ-AV40 CMV Strong promoter, broad scope of application. C-P2A-eGFP 2.6 kb
pEZ-AV41 EF1a Broad scope of application. Better choice than CMV for in vivo applications. C-P2A-eGFP 2.45 kb
pEZ-AV42 CAG Ubiquitous. Better choice than CMV for in vivo applications. C-P2A-eGFP 1.3 kb
pEZ-AV43 CMV Strong promoter, broad scope of application. N/A 3.45 kb
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-Y01 GAL1 Yeast N/A C-His N/A
pReceiver-YAD pADH Yeast N/A GAL4AD N/A
pReceiver-YBD pADH Yeast N/A GAL4DB N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-I01 AcMNPV polyhedrin Insect cell N/A N-His Tev
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-WG02 T7 cell free N/A N-His Factor Xa
pReceiver-WG03 T7 cell free N/A N-His-SUMO CoolCutter(TM)
pReceiver-WG04 T7 cell free N/A N-Avi-SUMO CoolCutter(TM)
pReceiver-WG05 T7 cell free N/A N-His-Avi-SUMO CoolCutter(TM)
pReceiver-WG09 T7 cell free N/A His-GST TEV
pReceiver-WG16 T7 cell free N/A N/A N/A
pReceiver-WG31 T7 cell free N/A N-His-SUMO-Avi CoolCutter(TM)
pReceiver-WG33 T7 cell free N/A N-Trx-His-SUMO CoolCutter(TM)
Catalog# Description
EX-EGFP-B01 Control vector (with EGFP) for pReceiver-B01
EX-EGFP-B02 Control vector (with EGFP) for pReceiver-B02
EX-EGFP-B03 Control vector (with EGFP) for pReceiver-B03
EX-EGFP-B04 Control vector (with EGFP) for pReceiver-B04
EX-EGFP-B05 Control vector (with EGFP) for pReceiver-B05
EX-EGFP-B06 Control vector (with EGFP) for pReceiver-B06
EX-EGFP-B07 Control vector (with EGFP) for pReceiver-B07
EX-EGFP-B08 Control vector (with EGFP) for pReceiver-B08
EX-EGFP-B09 Control vector (with EGFP) for pReceiver-B09
EX-EGFP-B10 Control vector (with EGFP) for pReceiver-B10
EX-EGFP-B11 Control vector (with EGFP) for pReceiver-B11
EX-EGFP-B12 Control vector (with EGFP) for pReceiver-B12
EX-EGFP-B13 Control vector (with EGFP) for pReceiver-B13
EX-EGFP-B31 Control vector (with EGFP) for pReceiver-B31
EX-EGFP-I01 Control vector (with EGFP) for pReceiver-I01
EX-EGFP-Lv105 Control vector (with EGFP) for pReceiver-Lv105
EX-EGFP-Lv151 Control vector (with EGFP) for pReceiver-Lv151
EX-EGFP-Lv152 Control vector (with EGFP) for pReceiver-Lv152
EX-EGFP-M01 Control vector (with EGFP) for pReceiver-M01
EX-EGFP-M02 Control vector (with EGFP) for pReceiver-M02
EX-EGFP-M04 Control vector (with EGFP) for pReceiver-M04
EX-EGFP-M05 Control vector (with EGFP) for pReceiver-M05
EX-EGFP-M06 Control vector (with EGFP) for pReceiver-M06
EX-EGFP-M07 Control vector (with EGFP) for pReceiver-M07
EX-EGFP-M08 Control vector (with EGFP) for pReceiver-M08
EX-EGFP-M09 Control vector (with EGFP) for pReceiver-M09
EX-EGFP-M10 Control vector (with EGFP) for pReceiver-M10
EX-EGFP-M11 Control vector (with EGFP) for pReceiver-M11
EX-EGFP-M12 Control vector (with EGFP) for pReceiver-M12
EX-EGFP-M13 Control vector (with EGFP) for pReceiver-M13
EX-EGFP-M14 Control vector (with EGFP) for pReceiver-M14
EX-EGFP-M17 Control vector (with EGFP) for pReceiver-M17
EX-EGFP-M48 Control vector (with EGFP) for pReceiver-M48
EX-EGFP-M49 Control vector (with EGFP) for pReceiver-M49
EX-EGFP-M50 Control vector (with EGFP) for pReceiver-M50
EX-EGFP-WG09 Control vector (with EGFP) for pReceiver-WG09
EX-EGFP-WG16 Control vector (with EGFP) for pReceiver-WG16
EX-EGFP-Y01 Control vector (with EGFP) for pReceiver-Y01
EX-EGFP-YAD Control vector (with EGFP) for pReceiver-YAD
EX-EGFP-YBD Control vector (with EGFP) for pReceiver-YBD
EX-NEG-Lv105 Empty control vector for pReceiver-Lv105
EX-NEG-Lv151 Empty control vector for pReceiver-Lv151
EX-NEG-Lv152 Empty control vector for pReceiver-Lv152
EX-NEG-M02 Empty control vector for pReceiver-M02
EX-NEG-M67 Empty control vector for pReceiver-M67
EX-NEG-M68 Empty control vector for pReceiver-M68
 

Largest number of tags to choose from

 
Fluorescent tags eGFP, eYFP, eCFP, mCherry
Multifunctional tags HaloTag®, AviTag™
Solubility and purification tags His, SUMO, GST, MBP, Flag, 3xFlag
Antibody Immunoprecipitation tags HA, cMyc
  Fusion tags table
  Learn More about ORF cDNA Clones Download – OmicsLink™ ORF cDNA clone brochure
 

miRNA Comprehensive Solutions

by | Mar 8, 2012 | Technologies

For studying the role of miRNA or trying to validate a putative target, GeneCopoeia has the right solutions. GeneCopoeia miRNA tools will help you design effective experiments to answer your questions.

  • What is the regulatory function of a specific miRNA? Do quantitative changes in its expression directly or indirectly lead to cancer or other diseases? If so, how much change?
  • How can I quickly detect and measure miRNA expression levels?
  • Which miRNA have an effect on my gene of interest?
  • Does a particular miRNA have the same influence on the same target gene in a different cell population?
  • Are there multiple targets for my miRNA? How can I validate a specific miRNA against a predicted target?
 
 

GeneCopoeia provides solutions for every step of miRNA functional analysis and detection

 

Detection and Quantitation of miRNA in cells

All-in-One™ miRNA qRT-PCR detection kits

All-in-One miRNA qRT-PCR Detection Kits combine PCR technology and SYBR Green in a fast and accurate quantitative analysis of mature miRNAs from total RNA for samples as small as 100 pg.

A novel optimized blend of poly A polymerase and reverse transcriptase with an RT buffer that was specifically developed for the All-in-One RT Mix delivers maximum activity of both enzymes for robust and reproducible data.

When used with All-in-One validated miRNA primers the result is high-performance gene-expression profiling of more than 700 human miRNAs.

Take advantage of the benefits of All-in-One for your miRNA detection.

  • Sensitive — detect miRNA in 100 pg of RNA
  • Practical — detect only mature miRNA
  • Compatible — combine with GeneCopoeia primers
  • Affordable — fits most budgets easily

 

All-in-One™ miRNA qPCR validated primers

The All-in-One qPCR miRNA-specific primers are designed by a proprietary algorithm and validated for more than 700 human miRNAs.

When used in combination with the All-in-One SYBR® Green miRNA qRT-PCR kit, the All-in-One miRNA primers deliver reliable and reproducible high performance in quantitative PCR assays.

 

miProfile™ miRNA qPCR Arrays

The miProfile™ miRNA qPCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells. They are built with validated primers and optimized reaction conditions for robust performance. 

Our miRNA qPCR arrays are available for profiling miRNome expression, cancer, and disease-related or focus-group miRNAs, as well as custom services.

 

Questions?  Contact us here or email directly at inquiry@genecopoeia.com.