Monthly Archives: July 2017

GLuc-ON™ Promoter Reporter Clones

Introduction How it works Related Products Resources Introduction Using a secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ promoter clones are designed for promoter analysis by detecting the real-time activities of over 20,000 human and 18,000 mouse promoters using live cell assays.GLuc-ON promoter clones can be ordered as pre-designed or custom-built in one of our robust vector systems. Currently we offer both single-reporter and dual-reporter vector systems. The single-reporter system uses GLuc, mCherry, or GFP as the promoter reporter. The......
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Product FAQs

ORF cDNA clones   shRNA clone sets   Genome editing products (Genome-TALER™ TALEN and TALE-TF custom services, Genome-CRISP™ CRISPR-Cas9 products and services, Human AAVS1 safe harbor gene targeting kits and clones)   Lentivirus (Lentifect™ Standard and Purified Lentivirus, Lenti-Pac™ Lentiviral Packaging Systems, lentiviral clones)   Stable cell lines   qPCR arrays and reagents   Protein expression   Luciferase assays   Fluorescent labeling and detection
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Luciferase Assays

Luciferase Assays Why is it advantageous to use a secreted reporter over Firefly or Renilla luciferases? Answer: To visualize Firefly and Renilla luciferase activity, it is necessary to first lyse the cells. On the other hand, GeneCopoeia’s Secrete-Pair™ Dual Luminescence Assay system uses two secreted reporters: Gaussia luciferase (GLuc) and secreted alkaline phosphatase (SEAP). Secreted reporters can be detected in the cell medium without lysing the cells. Therefore, you can assay the same sample multiple times, which is provides greater accuracy and reproducibility......
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Secrete-Pair™ Gaussia and SEAP Dual Luminescence Assay Kit

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a#button1:hover{ box-shadow: 0 1px 10px 0 rgba(0,0,0,0.24), 0 1px 10px 0 rgba(0,0,0,0.19) !important; background-color:#E1772F!important; }a#button2:hover{ box-shadow: 0 1px 10px 0 rgba(0,0,0,0.24), 0 1px 10px 0 rgba(0,0,0,0.19) !important; background-color:#0166B1!important; }ul{list-style-type:disc} Secrete-Pair™ Dual Luminescence Assay Kit – Gaussia Luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) dual-reporter system Secreted reporters enable live cell analysis. Ideal for time point studies and high-throughput ......
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Complete List of Cas9 Cell Lines

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a#button1:hover{ box-shadow: 0 1px 10px 0 rgba(0,0,0,0.24), 0 1px 10px 0 rgba(0,0,0,0.19) !important; background-color:#E1772F!important; }a#button2:hover{ box-shadow: 0 1px 10px 0 rgba(0,0,0,0.24), 0 1px 10px 0 rgba(0,0,0,0.19) !important; background-color:#0166B1!important; } Pre-made CRISPR-Cas9 Stable Cell Lines – Largest Collection On The Market Stable Cas9 integration eliminates need for the sgRNAs co-transfection. Ideal for high-throughput applications. Single clone isolation provides robust Cas9 expression in a unif......
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Lentifect™ Ultra-purified Lentivirus

For in vivo (animal) applications Overview Background and Data Related Products Technical Resources Overview GeneCopoeia’s Lentifect™ Ultra-purified Lentivirus is engineered for in vivo use, which is subjected to a multi-step purification process, resulting in high titers and ultra-high purity. This procedure ensures that viral preparations are free of conatminants that can cause undesired immunological responses and cytotoxicity, and is suitable for use in animal models. Lentifect™ custom ultra-purified lentivirus production services are available for ......
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Nextday-ORF™ Premade Expression-Ready ORF Clones

Next-day shipment / Starts from $69 GeneCopoeia's Nextday-ORF™ clones are collections of 140,000 premade open reading frame (ORF) clones that are expression-ready and available for immediate shipment. You don't have to wait for days or weeks to receive your ORF clones of interest and can start your gene overexpression or protein purification projects right away. Each Nextday-ORF™ clone is fully sequence-verified, ships next day*, and is available in multiple lentiviral or non-viral mammalian vector types. Advantages Next-day shipment* 140,000 premade ORF ......
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Application: Gene Expression and Cloning

Studying changes in gene expression and its consequences is a fundamental part of many areas of biomedical research, including diseases such as cancer and diabetes, as well as cell biology and agriculture. With molecular tools such as genome-wide expression-ready plasmid clones, reporter assays, and cloning kits, GeneCopoeia products cover nearly every conceivable application for Molecular Biology,   Clones & tag reagents For studying tagged and untagged proteins, promoter and miRNA elements, and genome editing. ......
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