Product Introduction
AAVPrime™ AAV Serotype Testing Kit
GeneCopoeia’s AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (Cat. No. AA320) contains 9 premade GFP-expressing AAV in serotypes 1, 2, 3, 6, 7, 8, 9, DJ and DJ/8.
AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit Plus (Cat. No. AA321) contains 12 premade GFP-expressing AAV in serotypes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, DJ and DJ/8. All AAV particles are offered at 5 ×1012 GC/ml, in 25 µL stocks, except serotypes 4 and 10 are offered at 5 ×1011~1012 GC/ml. These kits are ideal for testing the infection efficiency of AAV serotypes in different cell types.
Figure 1. AAV of different serotypes carrying GFP were used to transduce the H1299 cell line as shown in the figure above. Fluorescent images (GFP) show that cells are transduced with AAV-GFP.
AAVPrime™ AAV-qPCR Titration Kits
The AAV-qPCR Titration Kits are designed for quick and simple titration of Adeno-associated virus (AAV) particles using real-time quantitative PCR (qPCR), it can determines titration of all AAV serotypes as long as the ITR domains of the AAV genome is of AAV2 origin. The number of copies of the AAV genome is quantified using TaqMan –based qPCR.
The kits include the DNase I (RNase-free), AAV Lysis buffer, AAV-TaqMan qPCR mix (2x), AAV-Probe, and standard control.
AAV-293Ta packaging cell line
GCI-AAV-293Ta packaging cells for AAV production using optimized packaging plasmids with AAV vector-based constructs.
FAQs
Answer: All AAV serotypes are non-enveloped, single-stranded DNA parvoviruses. They share some properties including genome size, organization, and similar inverted terminal repeats. Despite similarities, different serotypes display significant differences in transduction efficiency and tissue tropism. AAV cellular entry is determined by its interactions with specific cell surface glycans and proteinaceous receptor(s). Different AAV serotypes have evolved distinctive interactions with the same receptor, therefore displaying various tissue tropism.
Answer:
| Serotype |
Primary Target Tissues |
Description |
| AAV-1 |
Muscle |
Best for cardiac muscle, skeletal muscle, neuronal and glial tissue. |
| AAV-2 |
Muscle, Liver, Retina |
Oldest and most commonly-used serotype. Best for neurons, muscle, liver, and brain. |
| AAV-3 |
Megakaryocytes |
Best for megakaryocytes, muscle, liver, lung, and retina. |
| AAV-4 |
Retina |
Best for neurons, muscle, brain, and retina. |
| AAV-5 |
Lung |
Best for lung, neurons, synovial joint, retina, and pancreas. |
| AAV-6 |
Muscle, Lung |
Best for lung, liver, and heart. |
| AAV-7 |
Muscle, Retina, Neurons |
Best for muscle, neurons, and liver. |
| AAV-8 |
Liver |
Best for muscle, brain, liver, and retina. |
| AAV-9 |
Various |
Best for muscle, heart, liver, lung, and brain. |
| AAV-10 |
Pleura, CNS |
Cloned from Cynomolgus, almost identical with AAVrh10 except for 12 amino acids in VP1. Best for lung, muscle, heart, CNS, and liver. |
| AAV-DJ |
Various |
A mixture of 8 naturally-occurring serotypes. Efficiently transduces a wide variety of cell types in vitro. |
| AAV-DJ/8 |
Various |
A variant of AAV-DJ with a mutation that permits infection of liver as well as other tissues in vivo. |
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