by admin | Sep 26, 2012 | FAQs
Answer: Currently, we only offer pre-made Human and Mouse ORF clones. We are in the process of producing full length ORF clones from other model animals such as rat. These full length ORF clones will be released to market in the near future. If you have full length ORF of any genes of interest from these model animals, please send us the information of these genes such as GenBank Accession or Nucleotide sequences. We will place them on the list of high priority Full length ORFs of genes for cloning into the vectors we offer. They will be charged with the same price as our other catalog ORF clones. We also offer custom cloning services for cloning genes from these two model animals into any vector(s) of your choice.
by admin | Sep 26, 2012 | FAQs
Answer: A correct DNA sequence with a promoter does not guarantee you to get protein expressed. Many factors contribute to the success or failure of protein expression 1) Did you use the right type of expression vector for your expression host system, for instance our pReceiver-M01 vector is for mammalian cell expression and pReceiver-B01 is for bacterial expression? 2) If the protein is a membranous protein, does it need target sequence for proper localization? 3) Will the over-expressed protein be toxic to the host? 4) Will the protein be folded into a right three-dimensional structure for its proper function and/or immunological-assays? 5) Have you checked the sequencing to see if you get an alternative splicing variant?
by admin | Sep 26, 2012 | FAQs
Answer: All expression clones in pReceiever-B01 and pReceiver-WG02/WG03/WG04/WG05/WG09/WG16 have been tested for expression in E. coli or cell free protein production system made by Roche. Other expression clones with different features and for different host cell systems have not been tested for protein expression.
by admin | Sep 26, 2012 | FAQs
Answer: We will make the best effort to clone the original splicing form and provide you with the replacement clone. However, we cannot guarantee that we will get the clone of a specific splicing variant.
by admin | Sep 26, 2012 | FAQs
Answer: Even though we cannot provide replacement clones for this type of cases, we do offer mutagenesis services to change any nucleotide compositions as requested for a nominal fee.
