Monthly Archives: May 2019

CRISPR-Cas9 Protein for Highly Efficient Genome Editing With Minimal Off-Target Activity

  Ed Davis, Ph.D. Introduction The Clustered, Regularly Interspaced, Short Palindromic Repeats (CRISPR) system is widely used for editing genes and modulating gene expression, thanks to its ease-of-design, efficiency, specificity, and relatively low cost (Wang, et al., 2016). CRISPR-based tools for gene editing are adapted from anti-viral immunity mechanisms present in many bacterial and archaea species. The most commonly-used CRISPR system to date employs the CRISPR-Cas9 nuclease from Streptococcus pyogenes (SpCas9). SpCas9 is a DNA endonuclease that, in the ......
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