Tn5 Transposase
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Introduction
Tn5 Transposase efficiently catalyzes the random insertion of Tn5 transposons into target sequences, exhibiting extremely high transposition insertion efficiency for both eukaryotic and prokaryotic DNA.
The enzyme specifically recognizes the inverted repeat Mosaic End (ME) sequences flanking the transposon. It recognizes DNA fragments bearing chimeric ME sequences at both ends (including primers containing ME sequences) and forms a Tn5 transposome.This transposome can randomly bind target DNA, cleave it, and insert the carried DNA fragment. Tn5 Transposase is widely used in in vitro transgenesis and next-generation sequencing (NGS) library preparation.
Figure 1. Schematic illustrating the working principle of Tn5 Transposase.
Applications
- Fragmentation and adapter addition for next-generation sequencing library preparation.
- Construction of random transposon insertion mutant libraries in vitro or in vivo.
- Rapid sequencing of large DNA molecules (e.g., BAC clones).
- Insertion of antibiotic resistance markers into target DNA.
