Monthly Archives: November 2019

EZShuttle™ Recombination Cloning System

  -Efficient cloning system based on phage lambda recombination, the same principle as Gateway® technology. Introduction EZRecombinase™ Expression vectors Shuttle clones Related products Publications Introduction The GeneCopoeia EZShuttle™ cloning system uses the site-specific recombination machinery between the E. coli and phage lambda genomes for efficient DNA fragment transfer between plasmids, the same principle as Gateway® technology. The EZShuttle™ system includes the following major components. Click on each tab above for more......
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Why You Should Know Your Gene’s Accession Number

  Ed Davis, Ph.D. Introduction A typical mammalian gene usually does not encode a single protein, thanks partly to the phenomenon of alternative mRNA splicing. Nearly all mammalian genes contain multiple introns, and greater than 90% of all intron-containing genes undergo alternative splicing to generate multiple transcript variants, and, subsequently, different protein isoforms (Pan, et al., 2008; Park, et al., 2018). Differential alternative splicing usually occurs within and between tissues, but some (5%) can occur between individuals (Kwan, et al., 2007; W......
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NileHiFi® Long Amplicon PCR Kit

GeneCopoeia's NileHiFi® Long Amplicon PCR Kit allows you to amplify templates up to 18kb of human genomic DNA (gDNA), 30kb of λ phage DNA, and other high molecular weight genomic DNA. This kit is recommended for amplifying GC rich or complex targets. The NileHiFi® Long Amplicon PCR Kit is the ideal choice for generating long PCR products with high sensitivity and processivity.  Features High-fidelity DNA polymerases with strong proofreading activity Efficiently amplify DNA templates up to 18 kb Compatible with blunt-end and TA c......
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NRAS Q61K Reference Standard

The NRAS Q61K Reference Standard is genomic DNA derived from a highly characterized heterozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation Q61K in NRAS on both chromosomes. The variant allele frequency (VAF) is 50%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {backgrou......
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PIK3CA E545K Reference Standard

The PIK3CA E545K Reference Standard is genomic DNA derived from a highly characterized heterozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation E545K in PIK3CA on both chromosomes. The variant allele frequency (VAF) is 50%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {ba......
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KRAS G13C Reference Standard

The KRAS G13C Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation G13C in KRAS on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {backgroun......
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EGFR V769-D770insASV Reference Standard

The EGFR V769-D770insASV Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation V769-D770insASV in EGFR on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .......
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EGFR L861Q Reference Standard

The EGFR L861Q Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation L861Q in EGFR on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {backgro......
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EGFR S768I Reference Standard

The EGFR S768I Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation S768I in EGFR on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {backgro......
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EGFR G719S Reference Standard

The EGFR G719S Reference Standard is genomic DNA derived from a highly characterized homozygous diploid HCT116 cell line, genetically modified with advanced genome editing technology to include the mutation G719S in EGFR on both chromosomes. The variant allele frequency (VAF) is 100%, and can be diluted using the corresponding wild-type standard to reach lower VAF. This reference standard can be used to assess the performance of your NGS, sanger sequencing and qPCR assay, including the limit of detection, accuracy, and precision. .accordion_h {backgro......
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