Monthly Archives: July 2016

EGR1 transcriptional response element (TRE) lentiviral clone

GeneCopoeia's GLuc-ON™ EGR1 transcriptional response element (TRE) lentiviral clone enables analysis of the effects on genes regulated by early growth respionse protein 1 (EGR1), such as  transforming growth factor-beta-1 (TGFB1) and fibronectin (FN1). EGR1 is a transcription factor that responds to mitogenic stimulation. EGR1 regulates many processes, such as neuron activity, wound healing, apopotosis, and angiogenesis, and is known to both suppress and promote tumor growth. The GLuc-ON™ PKC EGR1 lentiviral clone contains tandem repeats of EGR1 upstream of a minima......
Posted in Luciferase Assays and Reporters, Products

PKC transcriptional response element (TRE) lentiviral clone

GeneCopoeia's GLuc-ON™ PKC transcriptional response element (TRE) lentiviral clone enables analysis of the effects on genes regulated by activator protein 1 (AP1). AP1 is a transcription factor complex comprised of the c-Fos and c-Jun family proteins,  and is necessary for signaling through the Protein kinase C (PKC) pathway. The GLuc-ON™ PKC TRE lentiviral clone contains tandem repeats of PKC upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The PKC response eleme......
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TCF/LEF transcriptional response element (TRE) lentiviral clone

GeneCopoeia provides the GLuc-ON™ TCF/LEF transcriptional response element (TRE) lentiviral clone for analysis of the effects on Wnt signaling pathways mediated by TCF/LEF transcription factors, which recruit β-catenin to activate the promoters of several genes critical for function of the Wnt pathway. Wnt signaling plays fundamental roles in embryonic development, and its misregulation has been implicated in diseases such as cancer and type 2 diabetes. The GLuc-ON™ TCF/LEF TRE lentiviral clone contains tandem repeats of TCF/LEF upstream of a minimal promoter and secret......
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NFAT transcriptional response element (TRE) lentiviral clone

GeneCopoeia's GLuc-ON™ NFAT transcriptional response element (TRE) lentiviral clone enables analysis of calcium signaling pathways mediated by the nuclear factor of activated T cells (NFAT) family of transcription factors. NFATs, in combination with AP-1, regulate several genes, such as cytokines, in response to levels of intracellular calcium, and are important functional determinants of the immune sytem. The GLuc-ON™ NFAT TRE lentiviral clone contains tandem repeats of NFAT upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robus......
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CREB transcriptional response element (TRE) lentiviral clone

GeneCopoeia's GLuc-ON™ CREB transcriptional response element (TRE) lentiviral clone enables analysis of the effects on the cAMP and PKA signaling pathways through the cAMP response element (CRE). CRE, which is found in the promoters of many genes, including somatotatin, vegetative growth factor (VGF), and the protooncogene c-fos,becomes bound by the transcription factor CRE binding protein (CREB) in response to elevated cAMP levels. The GLuc-ON™ CREB TRE lentiviral clone contains tandem repeats of CRE upstream of a minimal promoter and secreted Gaussia luciferase reporte......
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NFκB transcriptional response element (TRE) Clone

GeneCopoeia's GLuc-ON™ NFκB transcriptional response element (TRE) clone enables analysis of the activity of NFκB-regulated signal transduction pathways. NFκB is a transcription factor with may gene targets, such as B-cell Activating Factor, T-cell secreted factor, and chemokines. NFκB plays critical roles in immune system function, and its misregulation can lead to inlammatory diseases and cancer. The GLuc-ON™ NFκB TRE clone contains tandem repeats of NFκB upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, w......
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LXRa transcriptional response element (TRE) Clone

GeneCopoeia's GLuc-ON™ LXRa transcriptional response element (TRE) clone enables analysis of the transcriptional activity of liver X receptor (LXR). LXRs comprise 2 members: LXRa and LXRb. LXRs are nucleus-localized transcription factors that bind oxidized forms of cholesterol, and are critical players in regulation of cholesterol levels. The GLuc-ON™ LXRa TRE clone contains tandem repeats of LXRa upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The LXRa response eleme......
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PR transcriptional response element (TRE) Clone

GeneCopoeia's GLuc-ON™ PR transcriptional response element (TRE) clone enables analysis of the activity of signal transduction mediated by progesterone receptors (PR). Progesterone receptors are transcription factors that, in response to stimulation by progesterone, lead to proliferation of breast tissue. Aberrant function and/or expression of PRs is an important factor in development of breast cancer The GLuc-ON™ PR TRE clone contains tandem repeats of PR upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive anal......
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PPAR transcriptional response element (TRE) Clone

GeneCopoeia's GLuc-ON™ PPAR transcriptional response element (TRE) clone enables analysis of transcriptional activity of peroxisome proliferator-activated receptors (PPARs).  PPARs are a family of three nucleus-localized transcription factors-PPARα, PPARβ/δ, and PPARγ that lead to peroxisome proliferation in response to lipid ligands. PPARs are important players in treatments of various diseases, such as obesity and diabetes. The GLuc-ON™ PPAR TRE clone contains tandem repeats of PPAR upstream of a minimal promoter and secreted Gaussia lucifer......
Posted in Luciferase Assays and Reporters, Products

GR transcriptional response element (TRE) Clone

GeneCopoeia‘s GLuc-ON™ GR transcriptional response element (TRE) clone enables analysis of gene activation in response to glucocorticoid receptor activity. The glucocorticoid receptor is a transcription factor that is transported to the nucleus in response to steroid hormone binding, and is involved in many physiological processes, such as stress, lipid metabolism, and inflammation. The GLuc-ON™ GR TRE clone contains tandem repeats of GR upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysi......
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