Introduction

GeneCopoeia’s GLuc-ON™ NFκB transcriptional response element (TRE) clone enables analysis of the activity of NFκB-regulated signal transduction pathways. NF-κB is a transcription factor with numerous gene targets, including B-cell Activating Factor, T-cell secreted factor, and chemokines. NFκB plays critical roles in immune system function, and its misregulation can lead to inflammatory diseases and cancer.

The GLuc-ON™ NFκB TRE clone contains tandem repeats of NFκB upstream of a minimal promoter and a secreted Gaussia luciferase reporter gene, allowing for robust and sensitive analysis without cell lysis.  The NFκB response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ NFκB TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activated) ,and normalization (secreted alkaline phosphatase) control clones are also available for purchase.

User Manual

Validation Data

Figure 1. Activation of the GLuc-ON™ NFκB TRE by TNF-γ. 293T cells were transfected with a negative control clone or the GLuc-ON™ NFκB TRE clone. Transfected cells were incubated for 24 hours, followed by 18 hours of treatment with TNF-γ. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.

Introduction

GeneCopoeia’s GLuc-ON™ LXRa transcriptional response element (TRE) clone enables analysis of the transcriptional activity of liver X receptor (LXR). LXRs comprise 2 members: LXRa and LXRb. LXRs are nucleus-localized transcription factors that bind oxidized forms of cholesterol and are critical players in the regulation of cholesterol levels.

The GLuc-ON™ LXRa TRE clone contains tandem repeats of LXRa upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The LXRa response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ LXRa TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activated) and normalization (secreted alkaline phosphatase) control clones are also available for purchase.

User Manual

 

Validation Data

    Figure 1. Activation of the GLuc-ON™ LXRa TRE by exogenous expression of LXRa. 293T cells were co-transfected with (left to right) negative TRE clone + negative expression clone, negative TRE clone + LXRa expression clone, LXRa TRE clone + negative expression clone, or LXRa TRE clone + LXRa expression clone. Transfected cells were incubated for 40 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.

Introduction

GeneCopoeia’s GLuc-ON™ PR transcriptional response element (TRE) clone enables analysis of the activity of signal transduction mediated by progesterone receptors (PR). Progesterone receptors are transcription factors that, in response to stimulation by progesterone, lead to the proliferation of breast tissue. Aberrant function and/or expression of PRs is an important factor in the development of breast cancer

The GLuc-ON™ PR TRE clone contains tandem repeats of PR upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The PR response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ PR TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activated), and normalization (secreted alkaline phosphatase) control clones are also available for purchase.

User Manual

Validation Data

Figure 1. Activation of the GLuc-ON™ PR TRE by exogenous expression of PR. 293T cells were were co-transfected in the presence of 10 nM progesterone with (left to right) negative TRE clone + negative expression clone, negative TRE clone + PR expression clone, PR TRE clone + negative expression clone, or PR TRE clone + PR expression clone. Transfected cells were incubated for 40 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.

Introduction

GeneCopoeia’s GLuc-ON™ PPAR transcriptional response element (TRE) clone enables analysis of transcriptional activity of peroxisome proliferator-activated receptors (PPARs).  PPARs are a family of three nucleus-localized transcription factors-PPARα, PPARβ/δ, and PPARγ that lead to peroxisome proliferation in response to lipid ligands. PPARs are important players in treatment of various diseases, such as obesity and diabetes.

The GLuc-ON™ PPAR TRE clone contains tandem repeats of PPAR upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The PPAR response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ PPAR TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activated) ,and normalization (secreted alkaline phosphatase) control clones are also available for purchase.

User Manual

 

Validation Data

Figure 1. Activation of the GLuc-ON™ PPAR TRE by exogenous expression of PPAR. 293T cells were were co-transfected in the presence of 1 µM rosiglitazone with (left to right) negative TRE clone + negative expression clone, negative TRE clone + PPAR expression clone, PPAR TRE clone + negative expression clone, or PPAR TRE clone + PPAR expression clone. Transfected cells were incubated for 40 hours. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.

Introduction

GeneCopoeia’s GLuc-ON™ GR transcriptional response element (TRE) clone enables analysis of gene activation in response to glucocorticoid receptor activity. The glucocorticoid receptor is a transcription factor that is transported to the nucleus in response to steroid hormone binding, and is involved in many physiological processes, such as stress, lipid metabolism, and inflammation.

The GLuc-ON™ GR TRE clone contains tandem repeats of GR upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The GR response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ GR TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreted alkaline phosphatase) control clones are also available for purchase.

User Manual

Validation Data

    Figure 1. Activation of the GLuc-ON™ GR TRE by dexamethasone (DEX). 293T cells were transfected with a negative control clone or the GLuc-ON™ GR TRE clone. Transfected cells were incubated for 24 hours, followed by 18 hours of treatment with DEX. A Gaussia  Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.